US2016376552A1PendingUtilityA1

Method for producing continuous cell lines

Assignee: NANOTHERAPEUTICS INCPriority: Feb 25, 2008Filed: Jul 11, 2016Published: Dec 29, 2016
Est. expiryFeb 25, 2028(~1.6 yrs left)· nominal 20-yr term from priority
C12N 5/0603C12N 15/01C12N 2770/24151C12N 2710/24151C12N 2510/04C12N 2760/16051C12N 7/00C12R 2001/91C12N 1/00C12N 2760/16151
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Claims

Abstract

The present invention relates to a method for production of continuous cell lines comprising providing living cells of an animal or a human, irradiating said cells with UV light, proliferating said cells and selecting multiplying cells as cells of a continuous cell line.

Claims

exact text as granted — not AI-modified
1 . A continuous avian cell line obtainable obtained by a method comprising irradiating isolated avian cells with an effective dose of ultraviolet (UV) light having a wavelength between 200 nm to 300 nm at a dose of from about 20 mJ/cm 2  to about 105 mJ/cm 2 , wherein the irradiation time is from 30 seconds to 4 minutes, and selecting cells that proliferate after at least 20 passages as cells of the continuous avian cell line. 
     
     
         2 . The continuous avian cell line of  claim 2 , wherein the dose of UV light is at least 50 mJ/cm 2 .

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