Medication of Suppressing Viability of Tumor Cells

Abstract

A method of culturing Antrodia cinnamomea and obtaining Antrodia cinnamomea with improved contents of antcin C and zhankuic acid C is disclosed in the invention. The method comprises: providing a wood segment of Cinnamomum kanehirai; inoculating a strain of Antrodia cinnamomea to the wood segment, to obtain the wood segment inoculated with the strain of Antrodia cinnamomea with 0.162 g dry weight of hyphae and mycelia of the strain of Antrodia cinnamomea per kg wood segment; and irradiating the wood segment inoculated with the strain of Antrodia cinnamomea by a light of wavelength of 600-700 nm to obtain a fruit body; wherein an irradiation time of the wood segment inoculated with the strain of Antrodia cinnamomea is 8-12 hours per day for 30-40 days.

What is claimed is: 
     
         1 . A medication of suppressing viability of hepatic tumor cells, comprising an extract of  Antrodia cinnamomea  having an elevated antcin C and zhankuic C content, wherein the extract of  Antrodia cinnamomea  is prepared by a method comprising the steps of:
 (a) providing a wood segment of  Cinnamomum kanehirai,      (b) inoculating 0.162 g of dry weight of hyphae and mycelia of a strain of  Antrodia cinnamomea  per kilogram of the wood segment to produce an inoculated wood segment,   (c) culturing the inoculated wood segment for 30-40 days under irradiation by light of wavelength 600-700 nm for 8-12 hours per day to obtain an  Antrodia cinnamomea  fruit body,   (d) extracting the fruit body under ultrasonic conditions with 95% ethanol at a temperature under 25° C. to produce said extract having an elevated antcin C and zhankuic C content.   
     
     
         2 . A medication of suppressing viability of lung tumor cells, comprising an extract of  Antrodia cinnamomea  having an elevated antcin C and zhankuic C content, wherein the extract of  Antrodia cinnamomea  is prepared by a method comprising the steps of:
 (a) providing a wood segment of  Cinnamomum kanehirai,      (b) inoculating 0.162 g of dry weight of hyphae and mycelia of a strain of  Antrodia cinnamomea  per kilogram of the wood segment to produce an inoculated wood segment,   (c) culturing the inoculated wood segment for 30-40 days under irradiation by light of wavelength 600-700 nm for 8-12 hours per day to obtain an  Antrodia cinnamomea  fruit body,   (d) extracting the fruit body under ultrasonic conditions with 95% ethanol at a temperature under 25° C. to produce said extract having an elevated antcin C and zhankuic C content.   
     
     
         3 . A medication of suppressing viability of colorectal tumor cells, comprising an extract of  Antrodia cinnamomea  having an elevated antcin C and zhankuic C content, wherein the extract of  Antrodia cinnamomea  is prepared by a method comprising the steps of:
 (a) providing a wood segment of  Cinnamomum kanehirai,      (b) inoculating 0.162 g of dry weight of hyphae and mycelia of a strain of  Antrodia cinnamomea  per kilogram of the wood segment to produce an inoculated wood segment,   (c) culturing the inoculated wood segment for 30-40 days under irradiation by light of wavelength 600-700 nm for 8-12 hours per day to obtain an  Antrodia cinnamomea  fruit body,   (d) extracting the fruit body under ultrasonic conditions with 95% ethanol at a temperature under 25° C. to produce said extract having an elevated antcin C and zhankuic C content.   
     
     
         4 . A medication of suppressing viability of sarcoma cells, comprising an extract of  Antrodia cinnamomea  having an elevated antcin C and zhankuic C content, wherein the extract of  Antrodia cinnamomea  is prepared by a method comprising the steps of:
 (a) providing a wood segment of  Cinnamomum kanehirai,      (b) inoculating 0.162 g of dry weight of hyphae and mycelia of a strain of  Antrodia cinnamomea  per kilogram of the wood segment to produce an inoculated wood segment,   (c) culturing the inoculated wood segment for 30-40 days under irradiation by light of wavelength 600-700 nm for 8-12 hours per day to obtain an  Antrodia cinnamomea  fruit body,   (d) extracting the fruit body under ultrasonic conditions with 95% ethanol at a temperature under 25° C. to produce said extract having an elevated antcin C and zhankuic C content.   
     
     
         5 . A medication of suppressing viability of breast tumor cells, comprising an extract of  Antrodia cinnamomea  having an elevated antcin C and zhankuic C content, wherein the extract of  Antrodia cinnamomea  is prepared by a method comprising the steps of:
 (a) providing a wood segment of  Cinnamomum kanehirai,      (b) inoculating 0.162 g of dry weight of hyphae and mycelia of a strain of  Antrodia cinnamomea  per kilogram of the wood segment to produce an inoculated wood segment,   (c) culturing the inoculated wood segment for 30-40 days under irradiation by light of wavelength 600-700 nm for 8-12 hours per day to obtain an  Antrodia cinnamomea  fruit body,   (d) extracting the fruit body under ultrasonic conditions with 95% ethanol at a temperature under 25° C. to produce said extract having an elevated antcin C and zhankuic C content.