Process for the purification of heparan sulfate and use thereof in cosmetological and dermatological preparations

Abstract

The present invention relates to cosmetological and dermatological compositions comprising heparan sulfate. The invention further discloses a process for the purification of heparan sulfate for dermatological and cosmetological applications, which process comprises the following steps: solubilization of heparan sulfate in water, adsorption on an anion exchange resin, desorption from the resin by using conditions which result in selective desorption of heparan sulfate. The cosmetological and dermatological compositions according to the invention show, inter alia, anti-age, lenitive, and whitening effect.

1 .- 13 . (canceled) 
     
     
         14 . A method of whitening skin, the method comprising applying a formulation comprising heparan sulfate to a patient in need thereof. 
     
     
         15 . The method of  claim 14 , wherein the formulation comprises from 0.01 to 5% by weight heparan sulfate. 
     
     
         16 . The method of  claim 14 , wherein the formulation comprises from 0.05 to 2% by weight heparan sulfate. 
     
     
         17 . The method of  claim 14 , wherein the formulation comprises from 0.1 to 1% by weight heparan sulfate. 
     
     
         18 . The method of  claim 14 , wherein the heparan sulfate has a molecular weight between 6,000 and 12,000 Da. 
     
     
         19 . The method of  claim 14 , wherein the formulation further comprises one or more of the following ingredients: preservatives, emulsifiers, stabilizers, humectants, anti-oxidants, and other components which are included in cosmetological and dermatological formulations. 
     
     
         20 . The method of  claim 14 , wherein the formulation is an emulsion. 
     
     
         21 . A method of reducing itching, the method comprising applying a formulation comprising heparan sulfate to a patient in need thereof. 
     
     
         22 . The method of  claim 21 , wherein the formulation comprises from 0.01 to 5% by weight heparan sulfate. 
     
     
         23 . The method of  claim 21 , wherein the formulation comprises from 0.05 to 2% by weight heparan sulfate. 
     
     
         24 . The method of  claim 21 , wherein the formulation comprises from 0.1 to 1% by weight heparan sulfate. 
     
     
         25 . The method of  claim 21 , wherein the heparan sulfate has a molecular weight between 6,000 and 12,000 Da. 
     
     
         26 . The method of  claim 21 , wherein the formulation further comprises one or more of the following ingredients: preservatives, emulsifiers, stabilizers, humectants, anti-oxidants, and other components which are included in cosmetological and dermatological formulations. 
     
     
         27 . The method of  claim 21 , wherein the formulation is an emulsion. 
     
     
         28 . A method for reducing free radicals as Reactive Oxygen Species (ROS) and increasing cell proliferation and protein synthesis, the method comprising applying a formulation comprising heparan sulfate to a patient in need thereof. 
     
     
         29 . The method of  claim 28 , wherein the formulation comprises from 0.01 to 5% by weight heparan sulfate. 
     
     
         30 . The method of  claim 28 , wherein the formulation comprises from 0.05 to 2% by weight heparan sulfate. 
     
     
         31 . The method of  claim 28 , wherein the formulation comprises from 0.1 to 1% by weight heparan sulfate. 
     
     
         32 . The method of  claim 28 , wherein the heparan sulfate has a molecular weight between 6,000 and 12,000 Da. 
     
     
         33 . The method of  claim 28 , wherein the formulation further comprises one or more of the following ingredients: preservatives, emulsifiers, stabilizers, humectants, anti-oxidants, and other components which are included in cosmetological and dermatological formulations.