Carotenoid Compound Coming From Plant And Containing Natural Astaxanthin, Preparation Method Therefor, And Composition

Abstract

The present invention provides a high-content carotenoid compound from adonis amurensis . The content of the total carotenoid of the carotenoid compound is greater than 95%. The content of all-trans (3S, 3′S)-carotenoid is greater than 80%. The carotenoid crystals have a high purity, and can be used in multiple forms in the fields of a dietary supplement of a human being, a food additive, a feed additive and a cosmetic product. In addition, the present invention also provides a method for manufacturing the compound.

We claim: 
     
         1 . A high-content carotenoid compound from flower, stem and fruit of  adonis amurensis , characterized in that, the content of the total carotenoid in the high-content carotenoid compound is higher than 95 wt %, wherein the proportion of all-trans (3S, 3′S)-astaxanthin in the total carotenoid is higher than 80 wt %, the all-trans (3S, 3′S)-astaxanthin has the molecular structure as shown in formula (I): 
       
         
           
           
               
               
           
         
       
     
     
         2 . The high-content carotenoid compound according to  claim 1 , characterized in that, the total carotenoid comprises semi-astacene, astacene and adonirubin. 
     
     
         3 . The high-content of carotenoid compound according to  claim 2 , characterized in that, the proportion of the total content of semi-astancene and astacene in the total carotenoid is less than 10 wt %, and the proportion of adonirubin in the total carotenoid is less than 10 wt %. 
     
     
         4 . A composition comprising the high-content carotenoid compound according to  claim 1 . 
     
     
         5 . The composition according to  claim 4 , characterized in that, further comprising one or more of vegetable oil, modified starch, sucrose, dextrin and gelatin. 
     
     
         6 . A composition comprising the high-content carotenoid compound according to  claim 2 . 
     
     
         7 . The composition according to  claim 6 , further comprising one or more of vegetable oil, modified starch, sucrose, dextrin and gelatin. 
     
     
         8 . A composition comprising the high-content carotenoid compound according to  claim 3 . 
     
     
         9 . The composition according to  claim 8 , further comprising one or more of vegetable oil, modified starch, sucrose, dextrin and gelatin. 
     
     
         10 . A method of preparing a high-content carotenoid compound by using  adonis amurensis  extract as raw material comprising the steps of:
 (a) mixing  Adonis amurensis  extract with alcohol(s) solvent and fully dissolving, to obtain a mixed solution;   (b) heating the mixed solution of step (a) to 25˜75° C., and dropwise adding an alkali solution to the reaction, and the reaction time is 0.5˜6.0 hr;   (c) adding a mixture of alcohols solvent and water to obtain a reaction solution, after completion of step (b);   (d) filtering the reaction solution of step (c) to obtain a filter cake, and then washing the filter cake with a mixture of alcohols solvent and water; and   (e) drying the filter cake of step (d) in vacuum to obtain a carotenoid compound;   wherein, in the high-content carotenoid compound, the content of the total carotenoid is higher than 95 wt %, the proportion of the all-trans (3S, 3′S) astaxanthin in the carotenoid is higher than 80 wt %, the all-trans (3S, 3′S)-astaxanthin has the molecular structure as shown in formula (I):   
       
         
           
           
               
               
           
         
       
     
     
         11 . The method according to  claim 10 , characterized in that, in step (a), the ratio of the volume of the alcohols solvent to the weight of  Adonis amurensis  extract is 5˜50 times, preferably, the ratio of the volume of the alcohols solvent to the weight of  Adonis amurensis  extract is 25˜40 times. 
     
     
         12 . The method according to  claim 10 , characterized in that, in step (b), the concentration of the alkali solution of the reaction is 0.01˜2.50 mol/L after adding dropwise the alkali solution, preferably is 1.00˜2.00 mol/L. 
     
     
         13 . The method according to  claim 10 , characterized in that, the alcohols solvent in step (a) is ethanol, propylene glycol or glycerol; and
 the alkali solution in step (b) is sodium hydroxide solution, potassium hydroxide solution or sodium methoxide solution.   
     
     
         14 . The method according to  claim 10 , characterized in that, the volume ratio of the alcohols solvent to water in step (c) is 1:5˜10; and
 the volume ratio of the alcohols solvent to water in the step (d) is 2˜3:1.