Increased t-cell tumor infiltration and eradication of metastases by mutant light

Abstract

Mutant LIGHT expressed in a tumor environment elicited high levels of chemokines and adhesion molecules, accompanied by massive infiltration of naïve T lymphocytes. Methods and compositions to elicit immune responses against tumors including tumor volume reduction and eradication of metastasis using mutant LIGHT are disclosed.

1 .- 39 . (canceled) 
     
     
         40 . A method of inducing tumor-specific T-cell generation to reduce or control metastasis, the method comprising:
 (a) introducing a mutant LIGHT protein or a fragment thereof into an individual at a tumor site, wherein the mutant LIGHT protein or fragment does not have a proteolytic site; and   (b) reducing or controlling metastasis by inducing T-cell generation.   
     
     
         41 . The method of  claim 40 , wherein the generated T-cells are activated within a tumor site. 
     
     
         42 . The method of  claim 40 , wherein the generated T-cells circulate in blood. 
     
     
         43 . The method of  claim 42 , wherein the circulating T-cells are cancer specific. 
     
     
         44 . The method of  claim 40 , wherein the mutant LIGHT protein or the fragment is introduced into a tumor cell in vitro and the tumor cell expressing the mutant LIGHT protein or the fragment is delivered to the individual. 
     
     
         45 . The method of  claim 40 , wherein the T-cell generation is CD8+ dependent.