Anti-glucagon receptor antibodies and methods of use thereof

Abstract

The present invention provides antagonizing antibodies that bind to glucagon receptor and methods of using same. The anti-glucagon receptor antibodies can be used therapeutically to lower glucose levels in blood, and can be in the prevention and/or treatment of glucose-related disorders, including diabetes, hyperglycemia, hyperinsulinemia, impaired fasting glucose, impaired glucose tolerance, dyslipidemia, or metabolic syndrome.

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         15 . An isolated cell line that produces an isolated antagonist antibody that specifically binds to glucagon receptor and comprises:
 a heavy chain variable region (VH) comprising a VH complementarity determining region one (CDR1), VH CDR2, and VH CDR3 of the VH having the amino acid sequence shown in SEQ ID NO: 11; and   a light chain variable region (VL) comprising a VL CDR1, VL CDR2, and VL CDR3 of the VL having the amino acid sequence shown in SEQ ID NO: 10.   
     
     
         16 . An isolated nucleic acid encoding tho antibody of  claim 1  an isolated antagonist antibody that specifically binds to glucagon receptor and comprises:
 a heavy chain variable region (VH) comprising a VH complementarity determining region one (CDR1), VH CDR2, and VH CDR3 of the VH having the amino acid sequence shown in SEQ ID NO: 11; and 
 a light chain variable region (VL) comprising a VL CDR1, VL CDR2, and VL CDR3 of the VL having the amino acid sequence shown in SEQ ID NO: 10. 
 
     
     
         17 . A recombinant expression vector comprising the nucleic acid of  claim 16 . 
     
     
         18 . A host cell comprising the expression vector of  claim 17 . 
     
     
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         20 . A method of producing an anti-glucagon receptor antagonist antibody, the method comprising: culturing the isolated cell line of  claim 15  under conditions wherein the antibody is produced; and recovering the antibody. 
     
     
         21 . A method of producing an anti-glucagon receptor antagonist antibody, the method comprising: culturing a cell line comprising nucleic acid encoding an antibody comprising a heavy chain comprising the amino acid sequence shown in SEQ ID NO: 87 or 88 and a light chain comprising the amino acid sequence shown in SEQ ID NO: 89 under conditions wherein the antibody is produced; and recovering the antibody. 
     
     
         22 . The method of  claim 21 , wherein the heavy and light chains are encoded on separate vectors. 
     
     
         23 . The method of  claim 21 , wherein the heavy and light chains are encoded on the same vector. 
     
     
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