Identification of secreted proteins as detection markers for citrus disease

Abstract

Secreted proteins as detection markers for insect vector and graft transmitted citrus disease are described. Method and kits for detecting the secreted proteins are provided.

What is claimed is: 
     
         1 . A kit for detecting citrus stubborn disease or detecting citrus greening disease (Huanglongbing or HLB), the kit comprising one or more reagent specific for a secreted protein from  Candidatus Liberibacter asiaticus , wherein the secreted protein consists of SEQ ID NO:29. 
     
     
         2 . The kit of  claim 1 , wherein the reagent is an antibody. 
     
     
         3 . The kit of  claim 2 , wherein the antibody is detectably-labeled. 
     
     
         4 . The kit of  claim 2 , wherein the antibody linked to a solid support. 
     
     
         5 . The kit of  claim 1 , wherein the reagent is an antibody that specifically binds to a protein consisting of SEQ ID NO:29. 
     
     
         6 . The kit of  claim 1 , wherein the kit further comprises a secondary antibody that binds to the reagent specific for the secreted protein. 
     
     
         7 . The kit of  claim 6 , wherein the secondary antibody is labeled with a direct or indirect label. 
     
     
         8 . The kit of  claim 6 , wherein the label is a direct label and the label is a fluorescent tag, luminescent tag, metal, dye, or radionucleotide linked to the secondary antibody. 
     
     
         9 . The kit of  claim 6 , wherein the label is an indirect label and the label is an enzyme. 
     
     
         10 . The kit of  claim 9 , wherein the enzyme is horseradish peroxidase (HRP), alkaline phosphatase (AP), β-galactosidase, or urease. 
     
     
         11 . The kit of  claim 1 , further comprising a solid support. 
     
     
         12 . The kit of  claim 11 , wherein the solid support is a magnetic particle, a chromatographic matrix particle, or the an assay plate. 
     
     
         13 . The kit of  claim 12 , wherein the assay plate is a microtiter plate. 
     
     
         14 . The kit of  claim 1 , wherein the reagent specific for a secreted protein is an antibody that specifically binds to a protein comprising SEQ ID NO:29, and the kit further comprises a secondary antibody linked to a direct or indirect label and a solid support. 
     
     
         15 . The kit of  claim 14 , wherein the secondary antibody that binds to the reagent specific for the secreted protein, wherein the secondary antibody is linked to an indirect label, which is an enzyme. 
     
     
         16 . The kit of  claim 14 , wherein the solid support is a microtiter plate. 
     
     
         17 . The kit of  claim 14 , wherein the kit further comprises a positive control. 
     
     
         18 . The kit of  claim 14 , wherein the kit further comprises a negative control.