Novel activity of ahpf protein of pseudomonas aeruginosa, and use therefor

Abstract

The present invention relates to an AhpF protein which has thioredoxin reductase, peroxidase, and chaperone activities and is derived from Pseudomonas aeruginosa , and a use therefor. By using a novel activity of the AhpF of Pseudomonas aeruginosa according to the present invention, it is possible to produce a plant having strong resistance to various environmental stresses such as oxidative stress or heat stress, thereby making it possible to contribute to increasing crop productivity and mass production of useful constituents. In addition, it is possible to prevent desertification and environmental pollution through the development of transformed plants having resistance to high temperatures and drying.

1 . An AhpF protein of  Pseudomonas aeruginosa  having thioredoxin reductase (TR), thioredoxine (Trx), peroxidase (Prx), and chaperone activities. 
     
     
         2 . The AhpF protein of  Pseudomonas aeruginosa  of  claim 1 , wherein it has an amino acid sequence of SEQ ID NO: 1. 
     
     
         3 . A high molecular weight complex of an AhpF protein of  Pseudomonas aeruginosa  characterized in that a holdase chaperone activity is increased and a molecular weight thereof is at least 500 to 2000 kDa, as a high molecular weight complex of the AhpF protein of  claim 1 . 
     
     
         4 . A low molecular weight complex of an AhpF protein of  Pseudomonas aeruginosa  characterized in that the peroxidase activity is increased and a molecular weight thereof is at least 100 to 250 kDa, as a low molecular weight complex of the AhpF protein of  claim 1 . 
     
     
         5 . A mutant of an AhpF protein of  Pseudomonas aeruginosa  in which cysteine residue at the position 342 in the amino acid sequence of SEQ ID No: 1 of  claim 2  is substituted with serine or alanine. 
     
     
         6 . A composition for improving resistance of organisms to environmental stress, the composition comprising a vector into which a polynucleotide encoding the AhpF protein of  Pseudomonas aeruginosa  of  claim 1  is introduced. 
     
     
         7 . The composition of  claim 6 , wherein the environmental stress is oxidative stress or heat stress. 
     
     
         8 . A bacterium in the  Agrobacterium  transformed by a recombinant vector expressing a gene of the AhpF protein of  Pseudomonas aeruginosa  of  claim 1 . 
     
     
         9 . A transgenic plant overexpressing the AhpF protein of  claim 1  to thereby have resistance to environmental stress. 
     
     
         10 . The plant of  claim 9 , wherein the plant is  Arabidopsis thaliana, Brassica rapa , or  Oryza sativa.    
     
     
         11 . A method of producing a plant having resistance to environmental stress, characterized in that the plant is transformed using a recombinant vector containing a polynucleotide encoding the AhpF protein of  claim 1 . 
     
     
         12 . The method of  claim 11 , wherein the recombinant vector is introduced into  Agrobacterium tumefaciens , thereby transforming the plant.