Wnt family-derived peptides and use thereof

Abstract

The present invention relates to a WNT-derived novel peptide and use thereof. WNT-derived peptide of the present invention possesses identical or similar activities to natural-occurring WNT protein, and has much higher stability and skin penetration potency than natural-occurring WNT protein. Therefore, the composition containing the present peptide not only shows excellent effects on improvement in hair loss (for example, promotion of hair growth or production of hair), but also has superior efficacies on treatment of a WNT signal transduction pathway-related disorder. In addition, the outstanding activity and stability of the present peptide described above may be greatly advantageous in application to pharmaceutical compositions, quasi-drugs and cosmetics.

1 . A peptide having a Wingless-type mouse mammary tumor virus integration site family (WNT) protein activity consisting of the amino acid sequence of SEQ ID NO:3. 
     
     
         2 . The peptide according to  claim 1 , wherein the peptide has at its N-terminal a protection group selected from the group consisting of acetyl group, fluorenyl methoxy carbonyl group, formyl group, palmitoyl group, myristyl group, stearyl group and polyethylene glycol (PEG). 
     
     
         3 . The peptide according to  claim 1 , wherein the peptide is derived from a human WNT protein. 
     
     
         4 . The peptide according to  claim 1 , wherein the peptide facilitates cell proliferation in fibroblasts, kerationcytes or hair follicle cells. 
     
     
         5 . The peptide according to  claim 1 , wherein the peptide promotes β-catenin signaling. 
     
     
         6 . The peptide according to  claim 1 , wherein the peptide promotes β-catenin signaling in spite of the presence of DKK-1 (Dickkopf-1) protein, which is involved in a hair loss process. 
     
     
         7 . The peptide according to  claim 1 , wherein the peptide promotes the expression of fibronectin, which is a target of WNT protein and important for hair growth. 
     
     
         8 - 15 . (canceled) 
     
     
         16 . A method for promoting hair growth or improving hair production comprising administering to a subject the peptide according to  claim 1 . 
     
     
         17 . A method for improving hair loss comprising administering to a subject the peptide according to  claim 1 . 
     
     
         18 . A method for improving skin conditions comprising administering to a subject the peptide according to  claim 1 . 
     
     
         19 . The method according to  claim 18 , wherein the improvement in the skin conditions is improvement in wrinkle or skin elasticity, prevention of skin aging, improvement in skin moisture, removal of wound or regeneration of skin. 
     
     
         20 . A method for preventing or treating a WNT signal transduction pathway-related disorder comprising administering to a subject the peptide according to  claim 1 . 
     
     
         21 . The method according to  claim 20 , wherein the WNT signal transduction pathway-related disorder comprises a bone disorder, osteoporosis or obesity. 
     
     
         22 . A method for preventing or treating a DKK-1 protein-related disorder, which is an antagonist of WNT signaling pathway, comprising administering to a subject the peptide according to  claim 1 . 
     
     
         23 . The method according to  claim 22 , wherein the DKK-1 protein-related disorder comprises insulin resistance, hypoinsulinemia, hyperinsulinemia, diabetes or obesity. 
     
     
         24 - 31 . (canceled)