Subgenomic replicons of the flavivirus dengue

Abstract

The present invention discloses the construction of dengue virus subgenomic replicons containing large deletions in the structural region (C-preM-E) of the genome, which replicons are useful as vaccines to protect against dengue virus infection.

1 - 24 . (canceled) 
     
     
         25 . A subgenomic replicon of dengue virus origin comprising a deletion for the sequence coding for PreM structural protein (ΔM). 
     
     
         26 . The subgenomic replicon of  claim 25 , wherein the dengue virus origin is dengue virus type 1, 2, 3, or 4 origin. 
     
     
         27 . The subgenomic replicon of  claim 25 , which is adapted to receive at least a nucleotide sequence without disrupting its replication capabilities. 
     
     
         28 . A vaccine or therapeutic comprising the subgenomic replicon of  claim 25  or  27  and a pharmaceutically acceptable carrier. 
     
     
         29 . A dengue virus like particle comprising a subgenomic replicon of dengue virus origin which comprises a deletion for the sequence coding for PreM structural protein (ΔM), optionally which is adapted to receive at least a nucleotide sequence without disrupting its replication capabilities, and structural proteins of the homologous dengue virus wherein said structural proteins encapsulate said subgenomic replicon. 
     
     
         30 . A method of immunization comprising administering to an individual in need thereof the subgenomic replicon of  claim 25 . 
     
     
         31 . A method of treatment comprising administering to an individual in need thereof the subgenomic replicon of  claim 25 .