Anti-fgfr4 antibodies and methods of use

Abstract

The invention provides anti-FGFR4 antibodies and methods of using the same.

1 - 41 . (canceled) 
     
     
         42 : An isolated nucleic acid encoding an anti-FGFR4 antibody heavy chain variable domain or light chain variable domain, wherein said antibody comprises (a) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1; (b) HVR-H2 comprising the amino acid sequence of SEQ ID NO:2; (c) HVR-H3 comprising the amino acid sequence of SEQ ID NO:3; (d) HVR-L1 comprising the amino acid sequence of SEQ ID NO:4; (e) HVR-L2 comprising the amino acid sequence of SEQ ID NO:5; and (f) HVR-L3 comprising the amino acid sequence of SEQ ID NO:6. 
     
     
         43 : The nucleic acid of  claim 42 , wherein the antibody further comprises a light chain variable domain framework sequence of SEQ ID NO: 9, 10, 11 and/or 12. 
     
     
         44 : The nucleic acid of  claim 42 , wherein the antibody further comprises a heavy chain variable domain framework sequence of SEQ ID NO: 12, 14, 15 and/or 16. 
     
     
         45 : The nucleic acid of  claim 42 , wherein the heavy chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 7 or the light chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 8. 
     
     
         46 : The nucleic acid of  claim 42 , wherein the nucleic acid encoding the heavy chain variable domain or light chain variable domain is on a separate vector from a nucleic acid encoding the corresponding light chain variable domain or heavy chain variable domain. 
     
     
         47 : The nucleic acid of  claim 42 , wherein the nucleic acid encoding the heavy chain variable domain or light chain variable domain and a nucleic acid encoding the corresponding light chain variable domain or heavy chain variable domain are on a single vector. 
     
     
         48 : The nucleic acid of  claim 42 , wherein the nucleic acid encodes the heavy chain variable domain and the light chain variable domain. 
     
     
         49 : The nucleic acid of  claim 42 , wherein the heavy chain variable domain sequence comprises the amino acid sequence of SEQ ID NO:7 and the light chain variable domain comprises the amino acid sequence of SEQ ID NO: 8. 
     
     
         50 : The nucleic acid of  claim 49 , wherein the nucleic acid encoding the heavy chain variable domain or light chain variable domain is on a separate vector from a nucleic acid encoding the corresponding light chain variable domain or heavy chain variable domain. 
     
     
         51 : The nucleic acid of  claim 49 , wherein the nucleic acid encoding the heavy chain variable domain or light chain variable domain and a nucleic acid encoding the corresponding light chain variable domain or heavy chain variable domain are on a single vector. 
     
     
         52 : The nucleic acid of  claim 42 , wherein said antibody is a full length IgG1 antibody. 
     
     
         53 : The nucleic acid of  claim 49 , wherein said antibody is a full length IgG1 antibody. 
     
     
         54 : A vector comprising the nucleic acid of  claim 42 . 
     
     
         55 : A vector comprising the nucleic acid of  claim 46 . 
     
     
         56 : A vector comprising the nucleic acid of  claim 48 . 
     
     
         57 : A vector comprising the nucleic acid of  claim 49 . 
     
     
         58 : A vector comprising the nucleic acid of  claim 50 . 
     
     
         59 : An isolated recombinant host cell transformed with the nucleic acid of  claim 42 . 
     
     
         60 : An isolated recombinant host cell transformed with the nucleic acid of  claim 46 . 
     
     
         61 : An isolated recombinant host cell transformed with the nucleic acid of  claim 48 . 
     
     
         62 : An isolated recombinant host cell transformed with the nucleic acid of  claim 49 . 
     
     
         63 : An isolated recombinant host cell transformed with the nucleic acid of  claim 50 . 
     
     
         64 : An isolated recombinant host cell transformed with the vector of  claim 54 . 
     
     
         65 : An isolated recombinant host cell transformed with the vector of  claim 58 . 
     
     
         66 : A method of making an anti-FGFR4 antibody comprising a heavy chain variable domain and a light chain variable domain, said method comprising culturing a recombinant host cell transformed with a first nucleic acid encoding the heavy chain variable domain and a second nucleic acid encoding the light chain variable domain, wherein said antibody comprises (a) HVR-H1 comprising the amino acid sequence of SEQ ID NO:1; (b) HVR-H2 comprising the amino acid sequence of SEQ ID NO:2; (c) HVR-H3 comprising the amino acid sequence of SEQ ID NO:3; (d) HVR-L1 comprising the amino acid sequence of SEQ ID NO:4; (e) HVR-L2 comprising the amino acid sequence of SEQ ID NO:5; and (f) HVR-L3 comprising the amino acid sequence of SEQ ID NO:6. 
     
     
         67 : The method of  claim 66 , wherein the heavy chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 7 and the light chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 8. 
     
     
         68 : A method of making an anti-FGFR4 antibody comprising a heavy chain variable domain and a light chain variable domain, said method comprising culturing a recombinant host cell transformed with a first vector comprising a nucleic acid encoding the heavy chain variable domain and a second vector comprising a nucleic acid encoding the light chain variable domain, wherein said antibody comprises (a) HVR-H1 comprising the amino acid sequence of SEQ ID NO: 1; (b) HVR-H2 comprising the amino acid sequence of SEQ ID NO:2; (c) HVR-H3 comprising the amino acid sequence of SEQ ID NO:3; (d) HVR-L1 comprising the amino acid sequence of SEQ ID NO:4; (e) HVR-L2 comprising the amino acid sequence of SEQ ID NO:5; and (f) HVR-L3 comprising the amino acid sequence of SEQ ID NO:6. 
     
     
         69 : The method of  claim 68 , wherein the heavy chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 7 and the light chain variable domain sequence comprises the amino acid sequence of SEQ ID NO: 8.