Lps vaccine

Abstract

A vaccine composition for birds comprising as an active ingredient a structure containing O-antigen derived from Gram-negative bacteria, provided that said structure does not contain a whole cell, and a process for preparing the same are provided. By using a structure containing O-antigen (e.g. lipopolysaccharide) derived from Gram-negative bacteria as an active ingredient in accordance with the present invention, alleviation of inoculation reaction and reduction in an amount of injection are attained as compared to the conventional whole-cells vaccine to thereby allow for the increase in the number of other antigens to be mixed therewith.

1 . A method of vaccinating a bird against  Salmonella , the method comprising administering to the bird, a composition comprising an O-antigen from  Salmonella , provided that said O-antigen is not comprised in a whole cell. 
     
     
         2 . The method of  claim 1 , wherein the  Salmonella  is one or more  Salmonella  groups selected from the group consisting of O, O7 and O9. 
     
     
         3 . The method of  claim 1 , wherein the composition comprises at least one purified lipopolysaccharide O-antigen from  Salmonella Enteritidis, Salmonella Typhimurium , and  Salmonella Infantis , provided that said O-antigen is not comprised in a whole cell. 
     
     
         4 . The method of  claim 1 , wherein the composition further comprises at least one of an antigen from Newcastle disease virus, an antigen from avian infectious bronchitis virus, an antigen from  Mycoplasma gallisepticum , an antigen from Egg drop syndrome virus, or an antigen from  Haemophilus paragallinarum.    
     
     
         5 . The method of  claim 1 , wherein the O-antigen comprises a lipopolysaccharide. 
     
     
         6 . The method of  claim 1 , wherein the composition comprises the O-antigen in an amount of at least 5,400 EU/ml. 
     
     
         7 . The method of  claim 1 , wherein the composition comprises the O-antigen in an amount of at least 54,000 EU/ml. 
     
     
         8 . The method of  claim 3 , wherein the composition comprises two of the purified lipopolysaccharide O-antigens. 
     
     
         9 . The method of  claim 3 , wherein the composition comprises three of the purified lipopolysaccharide O-antigens. 
     
     
         10 . The method of  claim 1 , wherein the bird is a chicken. 
     
     
         11 . The method of  claim 1 , wherein the composition further comprises an adjuvant. 
     
     
         12 . The method of  claim 1 , wherein the O-antigen is not bound to a carrier protein 
     
     
         13 . The method of  claim 1 , wherein the O-antigen is bound to a carrier protein. 
     
     
         14 . The method of  claim 13 , wherein the carrier protein is selected from the group consisting of diphtheria toxoid (DT), tetanus toxoid (TT), cholera toxin (CT) and CRM197.