Enhancement of electromagnetic freezing by stabilization and oscillation of biogenic magnetite particles

Abstract

Novel methods and systems for electromagnetic freezing are disclosed. An oscillating magnetic field can be applied in conjunction with a static magnetic field in order to align magnetic particles inside biological tissues. Such chains may be naturally present or artificially introduced. Needle-shaped electrodes may be used to produce ions and disturb the air layers insulating the tissues to be frozen.

What is claimed is: 
     
         1 . A method to freeze biological tissues, the method comprising:
 providing a refrigerating container configured to contain and freeze biological tissues, the refrigerating container having a plurality of needle-shaped electrodes pointing at the biological tissues, the needle-shaped electrodes being substantially close to, but separated from, the biological tissues;   aligning magnetic particles in the biological tissues by applying a static magnetic field to the biological tissues in the container;   vibrating the magnetic particles in the biological tissues in an elliptical or circular manner by applying an oscillating magnetic field to the biological tissues in the container, wherein the oscillating magnetic field is perpendicular to the static magnetic field with a component of rotation around it;   applying a voltage difference between the needle-shaped electrodes and the biological tissues, thereby producing ions in an air layer surrounding the biological tissues; and   freezing the biological tissues while the voltage difference and the static and oscillating magnetic fields are active.   
     
     
         2 . The method of  claim 1 , wherein an intensity of the static magnetic field is greater than 15 mT. 
     
     
         3 . The method of  claim 1 , wherein an intensity of the oscillating magnetic field is 10% of an intensity of the static magnetic field. 
     
     
         4 . The method of  claim 1 , further comprising introducing magnetic particles into the biological tissues prior to freezing. 
     
     
         5 . The method of  claim 4 , wherein the introducing is carried out by genetic activation of the biological tissues, thereby inducing the biological tissues to produce the magnetic particles.