Method For Enucleating Nucleated Erythrocyte, And Enucleation Inducer

Abstract

Provided is a factor capable of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time. More particularly, provided are a method of inducing enucleation, which is a final stage of erythrocyte differentiation, within a short time by adding a compound derived from proopiomelanocortin (POMC) to an undifferentiated (nucleated) erythrocyte, and an enucleation inducer including the compound.

1 . A method of enucleating a nucleated erythrocyte, comprising culturing a separated nucleated erythrocyte in a culture medium containing a compound derived from proopiomelanocortin (POMC). 
     
     
         2 . A method of enucleating a nucleated erythrocyte according to  claim 1 , wherein the compound is selected from any one of the following compounds:
 (1) POMC;   (2) ACTH;   (3) MSH;   (4) LPH;   (5) endorphin;   (6) CLIP; and   (7) a protected derivative, a glycosylated product, an acylated derivative, or an acetylated derivative of the compound according to any one of the items (1) to (6).   
     
     
         3 . A method of enucleating a nucleated erythrocyte according to  claim 1 , wherein the compound is selected from any one of the following compounds:
 (1) ACTH;   (2) α-MSH;   (3) CLIP; and   (4) a protected derivative, a glycosylated product, an acylated derivative, or an acetylated derivative of the compound according to any one of the items (1) to (3).   
     
     
         4 . A method of enucleating a nucleated erythrocyte according to  claim 1 , wherein the compound is selected from any one of the following peptides:
 (1) a peptide set forth in SEQ ID NO: 1;   (2) a peptide set forth in SEQ ID NO: 2;   (3) a peptide set forth in SEQ ID NO: 3;   (4) a peptide set forth in SEQ ID NO: 4;   (5) a peptide set forth in SEQ ID NO: 5;   (6) a peptide set forth in SEQ ID NO: 6;   (7) a peptide set forth in SEQ ID NO: 7;   (8) a peptide set forth in SEQ ID NO: 8;   (9) a peptide set forth in SEQ ID NO: 9;   (10) a peptide set forth in SEQ ID NO: 10;   (11) a peptide set forth in SEQ ID NO: 11;   (12) a peptide set forth in SEQ ID NO: 12;   (13) a peptide set forth in SEQ ID NO: 13;   (14) a peptide set forth in SEQ ID NO: 14;   (15) a peptide set forth in SEQ ID NO: 15;   (16) a peptide set forth in SEQ ID NO: 16;   (17) a peptide set forth in SEQ ID NO: 17;   (18) a peptide set forth in SEQ ID NO: 18;   (19) a peptide set forth in SEQ ID NO: 19;   (20) a peptide including the peptide according to any one of the items (1) to (19);   (21) a protected derivative, a glycosylated product, an acylated derivative, or an acetylated derivative of the peptide according to any one of the items (1) to (19);   (22) a peptide having 98% or more homology to the peptide according to any one of the items (1) to (19) and having substantially the same enucleation induction action on a nucleated erythrocyte as the peptide; and   (23) a peptide having 1 to 2 amino acid substitutions, deletions, insertions, and/or additions in the peptide according to any one of the items (1) to (19) and having substantially the same enucleation induction action on a nucleated erythrocyte as the peptide.   
     
     
         5 - 14 . (canceled) 
     
     
         15 . A method of enucleating a nucleated erythrocyte, comprising inducing enucleation in vitro through an action on MC1R, MC2R, MC3R, MC4R, and/or MC5R, which is a melanocortin receptor expressed on a nucleated erythrocyte.