Self-Assembling Oligonucleotides and Methods

Abstract

A composition comprising a double-stranded DNA filament having a plurality of single-species of oligonucleotide and chain terminator units, and a method for forming such filaments are disclosed. The oligonucleotide include a first segment, a second segment, pre-determined lengths, and pre-adjusted ratios of A:T/G:C between 1% and 50%. The chain terminator units include nucleotides of the first or second segment.

What is claimed is: 
     
         1 . A composition comprising a double-stranded DNA filament, the filament comprised of:
 a plurality of single-species of oligonucleotide, the oligonucleotide comprising:
 a first segment; 
 a second segment; 
 pre-determined lengths; and 
 pre-adjusted ratios of A:T/G:C between 1% and 50%; and 
   chain terminator units, the chain terminator units comprising:
 nucleotides of the first or second segment. 
   
     
     
         2 . The composition of  claim 1 , the single-species of oligonucleotide comprised of 8 to 26 oligonucleotides. 
     
     
         3 . The composition of  claim 1 , the first and second segments being palindromic to on another. 
     
     
         4 . The composition of  claim 1 , the first and second segments being self-complementary. 
     
     
         5 . The composition of  claim 1 , the first and second segments being the same length. 
     
     
         6 . The composition of  claim 1 , the first and second segments being of different length. 
     
     
         7 . The composition of  claim 1 , wherein each strand of the double-stranded DNA filament is substantially contiguous.