Method of lipid assay and reagent for use therein

Abstract

A method of lipid assay characterized by assaying the lipids contained in a blood component in the presence of an organic silicon compound. The method can cause specific conditions for direct methods while satisfying requirements such as no influence on precision of assay, no burden on assay apparatus, and easy availability.

1 . A method of assaying lipid characterized by assaying the lipid in blood components in the presence of an organic silicon compound. 
     
     
         2 . The method according to  claim 1 , wherein the organic silicon compound is a silicone and/or a derivative of silicone. 
     
     
         3 . The method according to  claim 1 , wherein the silicone or the derivative is selected from the group consisting of high polymerization silicone, cyclic silicone, silicone-containing surfactant, and modified silicone oil. 
     
     
         4 . The method according to  claim 1 , wherein the lipid component is a lipid contained in a specific lipoprotein. 
     
     
         5 . The method according to  claim 1 , wherein the lipid component is a cholesterol, triglycerides, or phospholipid. 
     
     
         6 . The method according to  claim 1 , wherein a lipid contained in a lipoprotein is detected by acting an enzyme on the lipoprotein under the conditions in which the organic silicon compound is present and a precipitation agent is not present. 
     
     
         7 . The method according to  claim 1 , wherein the enzyme is acted on the lipid in a lipoprotein in the presence of a surfactant having lipoprotein selectivity. 
     
     
         8 . A reagent for assaying lipids comprising an organic silicon compound. 
     
     
         9 . The reagent according to  claim 8 , wherein the organic silicon compound is a silicone and/or a derivative of silicone. 
     
     
         10 . The reagent according to  claim 8 , wherein the silicone or the derivative is selected from the group consisting of high polyinerization silicone, cyclic silicone, silicone-containing surfactant, and modified silicone oil. 
     
     
         11 . The reagent according to  claim 8 , wherein the lipid component is a lipid contained in a specific lipoprotein. 
     
     
         12 . The reagent according to  claim 8 , wherein the lipid component is a cholesterol, triglycerides, or phospholipid. 
     
     
         13 . A reagent for assaying HDL-cholesterol comprising (1) organic silicon compound, (2) cholesterol esterase, (3) cholesterol oxidase, (4) peroxidase, and (5) chromogen. 
     
     
         14 . A reagent according to  claim 13  further comprising a surfactant having lipoprotein selectivity. 
     
     
         15 . A kit for assaying LDL-cholesterol comprising:
 a first reagent which comprises (1) organic silicon compound, (2) cholesterol esterase, (3) cholesterol oxidase, (4) peroxidase, and (5) one of two compounds generating a color by combination, and   a second reagent which comprises (6) the other of the two compounds generating a color by combination and (7) surfactant with low lipoprotein selectivity.   
     
     
         16 . The kit for assaying LDL-cholesterol according to  claim 15 , wherein the first reagent further comprises a surfactant having lipoprotein selectivity.