Recombinant proteins and their therapeutic uses

Abstract

A recombinant protein expressing one or more human growth factors, tumor antigens, and/or receptors or epitopes thereof on or within an immunogenic expression creating a recombinant protein in which one or more epitopes are presented on the surface of the sequence in their natural configuration. The growth factor, tumor antigen, and/or receptor, sequence(s) may be expressed within the encoding sequence at appropriate internal positions or at the termini as single expressions or as two or more tandem repeats.

1 .- 33 . (canceled) 
     
     
         34 . A recombinant protein, comprising:
 a polypeptide sequence; and   a first sequence expressing at least a portion of a receptor along said polypeptide sequence.   
     
     
         35 . The recombinant protein according to  claim 34 , wherein said polypeptide sequence includes an immunogenic polypeptide sequence. 
     
     
         36 . The recombinant protein according to  claim 34 , wherein said polypeptide sequence includes a cholera toxin B (CT-B) protein. 
     
     
         37 . The recombinant protein according to  claim 34 , wherein said at least said portion of said receptor includes a full length or part thereof of one or more of the following receptors including but not limited to Human Epidermal growth factor Receptor 2 (Her2), Human Epidermal growth factor Receptor 3 (Her3), and other receptors. 
     
     
         38 . The recombinant protein according to  claim 37 , wherein said at least said portion of said receptor includes a full length or a portion of two to four different receptors present in said synthetic protein. 
     
     
         39 . The recombinant protein according  claim 37 , wherein said at least said portion of said receptor includes a full length or a portion of one or more receptors in said synthetic protein as single epitopes or as two or more tandem repeats. 
     
     
         40 . The recombinant protein according to  claim 34 , further comprising a second sequence expressing at least a portion of a growth factor along said polypeptide sequence. 
     
     
         41 . The recombinant protein according to  claim 40 , wherein said at least said portion of said growth factor includes a full length or part thereof of one or more of the following growth factors including but not limited to IGF-1, IGF-2, FGF1, FGF2, TGF-α, TGF-β, VEGF-A, VEGF-B, VEGF-C, VEGF, D, PDGF, NGF, EGF, HGF, BMP's, and IL's 1-6. 
     
     
         42 . The recombinant protein according to  claim 41 , wherein said at least said portion of said growth factor includes a full length or neutralizing portion of two to four different growth factors present in said synthetic protein. 
     
     
         43 . The recombinant protein according  claim 41 , wherein said at least said portion of said growth factor includes a full length or neutralizing portion of one or more growth factors in said synthetic protein as single epitopes or as two or more tandem repeats. 
     
     
         44 . The recombinant protein according  claim 41 , wherein said at least said portion of said growth factor or a neutralizing portion thereof is separated from the remaining synthetic protein by a peptide ‘spacer’. 
     
     
         45 . The recombinant protein according  claim 44 , wherein said peptide ‘spacer’ comprises in part a growth factor or neutralizing portion thereof. 
     
     
         46 . The recombinant protein according  claim 44 , wherein said peptide ‘spacer’ includes one or more T-cell epitopes. 
     
     
         47 . The recombinant protein according to  claim 40 , further comprising a third sequence expressing at least a portion of a tumor antigen along said polypeptide sequence. 
     
     
         48 . The recombinant protein according to  claim 47 , wherein said tumor antigen is a Prostate Specific Antigen (PSA). 
     
     
         49 . The recombinant protein according to  claim 47 , wherein said at least said portion of said tumor antigen includes a full length or part thereof of one or more of the following tumor antigens including but not limited to PSA, and other tumor antigens. 
     
     
         50 . The recombinant protein according to  claim 49 , wherein said at least said portion of said tumor antigen includes a full length or a portion of two to four different tumor antigens present in said synthetic protein. 
     
     
         51 . The recombinant protein according  claim 49 , wherein said at least said portion of said tumor antigen includes a full length or a portion of one or more tumor antigens in said synthetic protein as single epitopes or as two or more tandem repeats. 
     
     
         52 .- 54 . (canceled)