High-purity steviol glycosides

Abstract

Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Stevia rebaudiana Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.

1 - 26 . (canceled) 
     
     
         27 . A consumable product comprising Rebaudioside X made by converting Rebaudioside D to Rebaudioside X using a UDP-glucosyltransferase, wherein at least about 50% of the Rebaudioside D has been converted to Rebaudioside X. 
     
     
         28 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 50% by weight. 
     
     
         29 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 60% by weight. 
     
     
         30 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 70% by weight. 
     
     
         31 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 80% by weight. 
     
     
         32 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 90% by weight. 
     
     
         33 . The consumable product of  claim 27 , wherein the Rebaudioside X has a purity of greater than about 95% by weight.