System and method to divide liposuction fat into aliquots to be use and cryopreserved

Abstract

A system ( 1 ) to divide liposuction fat into aliquots for use and cryopreservation purposes, comprising: at least a transfer circuit ( 4, 10, 11 ) able to transfer sealed an adipose material removed by means of liposuction and comprising fat and an aqueous fluid, at least a taking container ( 12 ) which contains sealed the adipose material, syringe pumping means ( 2, 3 ), connected to the transfer circuit ( 4, 10, 11 ) and to the taking container ( 12 ), able to alternately take and contain sealed the adipose material from the taking container ( 12 ) and to transfer sealed the fat or said aqueous fluid within the circuit ( 4, 10, 11 ), at least a rejection receptacle ( 5 ) connected sealed to the circuit ( 4, 10, 11 ), downstream of the pumping means ( 2, 3 ), and able to receive the aqueous fluid, and a plurality of cryopreservation containers ( 6 ) to receive the fat in a sealing manner, connected to the circuit ( 4, 10, 11 ), downstream of the pumping means ( 2, 3 ), wherein the circuit ( 4, 10, 11 ) comprises first valve means ( 4 ) for allowing alternately the communication between the pumping means ( 2, 3 ) and the rejection receptacle ( 5 ) and between the pumping means and the cryopreservation container ( 6 ), wherein the syringe pumping means ( 2, 3 ) comprise a pumping syringe ( 2 ) and second valve means ( 3 ) able to put in sealed communication, alternately, the syringe ( 2 ) with the taking container ( 12 ) or the syringe ( 2 ) with the first valve means ( 4 ), and where the first valve means comprise a ramp ( 4 ) of three-way cocks, each individually connected either to a rejection receptacle ( 5 ) or to a cryopreservation container ( 6 ).

1 ) System ( 1 ) to divide liposuction fat into aliquots for use and cryopreservation purposes, wherein it comprises:
 at least a transfer circuit ( 4 ,  10 ,  11 ) able to transfer sealed an adipose material removed by means of liposuction and comprising fat and an aqueous fluid;   at least a taking container ( 12 ) which contains sealed said adipose material;   syringe pumping means ( 2 ,  3 ), connected to said transfer circuit ( 4 ,  10 ,  11 ) and to said taking container ( 12 ), able to alternately take and contain sealed said adipose material from said taking container ( 12 ) and to transfer sealed said fat or said aqueous fluid within said circuit ( 4 ,  10 ,  11 ); said pumping means ( 2 ,  3 ) being available so as to separate, by gravity, the fat from the aqueous fluids composing the adipose material contained in them;   at least a rejection receptacle ( 5 ) connected sealed to said circuit ( 4 ,  10 ,  11 ), downstream of said pumping means ( 2 ,  3 ), and able to receive said aqueous fluid; and   a plurality of cryopreservation containers ( 6 ) to receive said fat in a sealing manner, connected to said circuit ( 4 ,  10 ,  11 ), downstream of said pumping means ( 2 ,  3 );   wherein said circuit ( 4 ,  10 ,  11 ) comprises first valve means ( 4 ) for allowing alternately the communication between said pumping means ( 2 ,  3 ) and said rejection receptacle ( 5 ) and between said pumping means and said cryopreservation container ( 6 ),   wherein said syringe pumping means ( 2 ,  3 ) comprise a pumping syringe ( 2 ) and second valve means ( 3 ) able to put in sealed communication, alternately, said syringe ( 2 ) with said taking container ( 12 ) or said syringe ( 2 ) with said first valve means ( 4 ),   and where said first valve means comprise a ramp ( 4 ) of three-way cocks, each individually connected either to a rejection receptacle ( 5 ) or to a cryopreservation container ( 6 ).   
     
     
         2 ) A system ( 1 ) according to  claim 1 , wherein it comprises at least an access ( 7 ,  8 ) for the injection of a cryoprotective substance able to be put in communication with the inside of said cryopreservation container ( 6 ), the latter being made of a material resistant to a cryoprotective substance. 
     
     
         3 ) A system ( 1 ) according to  claim 2 , wherein said circuit ( 4 ,  10 ,  11 ) and said pumping means ( 2 ,  3 ) are made of a material resistant to a cryoprotective substance and in that said first valve means ( 4 ) are connected to said access ( 7 ) and are configured so as to also allow the sealed communication between said access ( 7 ) and said cryopreservation container ( 6 ). 
     
     
         4 ) A system ( 1 ) according to  claim 3 , wherein said cryoprotective substance comprises dimethyl sulfoxide and said material is resistant to dimethyl sulfoxide. 
     
     
         5 ) A system ( 1 ) according to  claim 2 , wherein said access ( 8 ) is obtained at the outer periphery of said cryopreservation container ( 6 ). 
     
     
         6 ) A method to divide liposuction fat into aliquots for use and cryopreservation purposes, wherein it comprises the following stages:
 providing a taking container ( 12 ) that contains adipose material removed by means of liposuction;   taking a quantity of said material from said taking container ( 12 ), keeping it isolated from the external environment;   separating the fat from the aqueous fluids composing the adipose material of said taken quantity; and   transferring in a sealing manner said separated fat into one or more cryopreservation containers ( 6 ), to define as many isolated aliquots of fat.   
     
     
         7 ) A method according to  claim 6 , wherein a predefined quantity of mesenchymal cells is added to said adipose material. 
     
     
         8 ) A. method according to  claim 6 , wherein said cryopreservation container/s ( 6 ) is/are made of a material resistant to a cryoprotective substance, and in that it comprises the stage of adding said cryoprotective substance to each of said aliquots of fat. 
     
     
         9 ) A method according to  claim 8 , wherein said cryoprotective substance comprises dimethyl sulfoxide, and that said material is resistant to dimethyl sulfoxide. 
     
     
         10 ) A method according to  claim 6 , wherein each cryopreservation container ( 6 ) is put in a refrigerator so that the respective aliquot of fat is preserved therein.