US2016168547A1PendingUtilityA1

Polypeptides Having Lipase Activity and Polynucleotides Encoding Same

60
Assignee: NOVOZYMES INCPriority: Sep 30, 2004Filed: Feb 23, 2016Published: Jun 16, 2016
Est. expirySep 30, 2024(expired)· nominal 20-yr term from priority
Inventors:Suzanne OtaniDebbie YaverHaiyan GeJanine LinChristopher AmoloKim BorchShamkant Anant PatkarMichael LamsaBarbara Cherry
C12N 9/20C12Y 301/01003
60
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Claims

exact text as granted — not AI-modified
1 . An isolated polypeptide having lipase activity, selected from the group consisting of:
 (a) a polypeptide having an amino acid sequence which has at least 60% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16;   (b) a polypeptide which is encoded by a polynucleotide which hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, or SEQ ID NO: 15, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, or SEQ ID NO: 15, or (iii) a complementary strand of (i) or (ii); and   (c) a variant comprising a conservative substitution, deletion, and/or insertion of one or more amino acids of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16.   
     
     
         2 . The polypeptide of  claim 1 , having an amino acid sequence which has at least 60% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         3 . The polypeptide of  claim 2 , having an amino acid sequence which has at least 65% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         4 . The polypeptide of  claim 3 , having an amino acid sequence which has at least 70% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         5 . The polypeptide of  claim 4 , having an amino acid sequence which has at least 75% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         6 . The polypeptide of  claim 5 , having an amino acid sequence which has at least 80% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         7 . The polypeptide of  claim 6 , having an amino acid sequence which has at least 85% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         8 . The polypeptide of  claim 7 , having an amino acid sequence which has at least 90% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         9 . The polypeptide of  claim 8 , having an amino acid sequence which has at least 95% identity with the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         10 . The polypeptide of  claim 1 , which comprises the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16; or a fragment thereof having lipase activity. 
     
     
         11 . The polypeptide of  claim 10 , which comprises the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         12 . The polypeptide of  claim 11 , which comprises the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         13 . The polypeptide of  claim 1 , which consists of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16; or a fragment thereof having lipase activity. 
     
     
         14 . The polypeptide of  claim 13 , which consists of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         15 . The polypeptide of  claim 13 , which consists of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16. 
     
     
         16 - 48 . (canceled) 
     
     
         49 . A method for preparing a dough or a baked product comprising incorporating into a dough a polypeptide of  claim 1 .

Join the waitlist — get patent alerts

Track US2016168547A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.