Regulation of Brain Biogenic Amines Associated with Depression by a Formulation from Botanical Source

Abstract

Provided herein are anti depressant and anxiolytic herbal formulations including: 225-450 mg of Nyctanthes arbortristis , 250-550 mg Ocimum tenuiflorum , 200-450 mg Hippophae salcifolia , and optionally additives selected from minerals, vitamins, salt, filler and binder. Also provided are methods of making such formulations and methods of using such formulations for treating or improving brain function.

1 . An anti depressant and anxiolytic herbal formulation comprising:
 225-450 mg of  Nyctanthes arbortristis;      250-550 mg  Ocimum tenuiflorum;      200-450 mg  Hippophae salcifolia ; and   optionally additives selected from minerals, vitamins, salt, filler and binder.   
     
     
         2 . The herbal formulation as claimed in  claim 1 , wherein the said formulation comprises whole plant of  Nyctanthes arbortristis , whole plant of  Ocimum tenuiflorum  and only leaves and fruits of  Hippophae salcifolia.    
     
     
         3 . The herbal formulation as claimed in  claim 1  wherein the amount of each of the components are:
 325 mg  Nyctanthes arbortristis;    
 350 mg of  Ocimum tenuiflorum;    
 275 mg of  Hippophae salcifolia.    
 
     
     
         4 . A process for the preparation of a herbal formulation comprising:
 grinding a portion of each of  Nyctanthes arbortristis, Ocimum tenuiflorum , and  Hippophae salcifolia , to provide ground herbal matter,   subjecting the ground herbal matter to the step of extraction using water to provide a:   filtering the extracts to provide a filtered product;   subjecting the filtered product to the step of distillation under vacuum;   evaporating the water and drying the filtered product under vacuum; and   grounding and sieving the filtered product.   
     
     
         5 . The process as claimed in  claim 4 , wherein the temperature at time of extraction is 70°-80° C. and the extraction process lasts for a period of 3-4 hrs. 
     
     
         6 . The process as claimed in  claim 4 , wherein the temperature of distillation is 65° C. 
     
     
         7 . (canceled) 
     
     
         8 . (canceled) 
     
     
         9 . A method of treating depression in a patient comprising administering to a patient in need thereof a formulation containing extract of  Nyctanthes arbortristis  and  Hippophae salciofolia  in an amount effective to promote sleep pattern and improve wake-cycle and depression symptoms. 
     
     
         10 . (canceled) 
     
     
         11 . (canceled) 
     
     
         12 . A method of treating depression in a patient comprising administering to a patient in need thereof a formulation comprising  Ocimum tenuiflorum  and  Hippophae salcifolia  in an amount effective to ameliorate symptoms of depression in the patient. 
     
     
         13 . The method of  claim 12 , wherein administering the formulation improves brain functional impairment, maintains hippocampal volume, and/or enhances neuronal plasticity in the patient. 
     
     
         14 . The method of  claim 12 , wherein administering the formulation improves sleep quality in the patient. 
     
     
         15 . A method of treating depression in a patient comprising administering to a patient in need thereof a formulation comprising  Nyctanthes arbortrist  and  Ocimum tenuiflorum  in an amount effective to ameliorate symptoms of depression in the patient. 
     
     
         16 . The method of  claim 15 , wherein administering the formulation reduces anxiety and/or phobic reactions in the patient. 
     
     
         17 . The method of  claim 15 , wherein administering the formulation improvise diffusion and hallucinations in the patient. 
     
     
         18 . The method of  claim 15 , wherein administering the formulation enhances immunity and/or improves fatigue in the patient.