Maspin, Maspin Derivatives, and Maspin Mimetics for Reducing ROS, Inflammation, and Skin Aging

Abstract

The present invention provides compositions, methods, and systems for treating inflammatory conditions (e.g., by inhibiting reactive oxygen species) in or on a subject with maspin, maspin derivatives, or maspin mimetics. In some embodiments, such agents are applied to the skin of a subject (e.g., to reduce skin aging).

We claim: 
     
         1 . A method of treating an inflammatory condition and/or skin aging comprising: administering to a subject a composition comprising maspin, a maspin derivative, or a maspin mimetic. 
     
     
         2 . The method of  claim 1 , wherein said administering is to the skin of said subject. 
     
     
         3 . The method of  claim 2 , wherein said composition further comprises skin lotion, cream, gel, or an ointment, a spray, or is present in a skin patch. 
     
     
         4 . The method of  claim 1 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:1-4. 
     
     
         5 . The method of  claim 1 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:5-7. 
     
     
         6 . The method of  claim 1 , wherein said maspin derivative comprises the amino acid sequence in SEQ ID NO:8. 
     
     
         7 . The method of  claim 6 , wherein said maspin derivative further comprises a peptide amphiphile. 
     
     
         8 . The method of  claim 1 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:9-12. 
     
     
         9 . The method of  claim 1 , wherein said maspin is full length or at least 99% of full-length human maspin. 
     
     
         10 . The method of  claim 1 , wherein said subject has, or is suspected of having, symptoms of an inflammatory condition selected from the group consisting of: benign prostate hyperplasia (BPH), inflammatory bowel disease (IBD), ROS-induced obesity, diabetes, and atherosclerosis. 
     
     
         11 . The method of  claim 10 , wherein said administering reduces or eliminates at least one of said symptoms of said inflammatory condition. 
     
     
         12 . A composition comprising: i) a human skin moisturizing lotion, get, cream, or ointment, and ii) maspin, a maspin derivative, or a maspin mimetic. 
     
     
         13 . The composition of  claim 12 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:1-4. 
     
     
         14 . The composition of  claim 12 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:5-7. 
     
     
         15 . The composition of  claim 12 , wherein said maspin derivative comprises the amino acid sequence in SEQ ID NO:8. 
     
     
         16 . The composition of  claim 15 , wherein said maspin derivative further comprises a peptide amphiphile. 
     
     
         17 . The composition of  claim 12 , wherein said maspin derivative comprises an amino acid sequence selected from SEQ ID NOs:9-12. 
     
     
         18 . The composition of  claim 12 , wherein said maspin is full length or at least 99% of full-length human maspin. 
     
     
         19 . A system comprising: i) a human skin moisturizing lotion, cream, gel, or ointment, and ii) maspin, a maspin derivative, or a maspin mimetic. 
     
     
         20 . The system of  claim 19 , wherein said maspin derivative comprise an amino acid sequence selected from SEQ ID NOs:1-13.