Proteases

Abstract

Proteases derived from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43235, Nocardiopsis prasina DSM 15649, Nocardiopsis prasina (previously alba ) DSM 14010 Nocardiopsis sp. DSM 16424, Nocardiopsis alkaliphila DSM 44657 and Nocardiopsis lucentensis DSM 44048, as well as homologous proteases; their recombinant production in various hosts, including transgenic plants and non-human animals, and their use in animal feed and detergents. The proteases are acid-stable, alkali-stable, and/or thermostable.

1 - 20 . (canceled) 
     
     
         21 . An isolated nucleic acid sequence comprising a nucleic acid sequence which encodes a polypeptide having protease activity, wherein the polypeptide is selected from the group consisting of:
 (a) a polypeptide having a sequence identity of at least 90% to the sequence of amino acids 1-192 of SEQ ID NO: 2, or which is a fragment of the sequence of amino acids 1-192 of SEQ ID NO: 2, that has protease activity;   (b) a polypeptide having a sequence identity of at least 90% to the sequence of amino acids 1-192 of SEQ ID NO: 8, or which is a fragment of the sequence of amino acids 1-192 of SEQ ID NO: 8, that has protease activity;   (c) a polypepdite having a sequence identity of at least 90% to the sequence of amino acids 1-189 of SEQ ID NO: 10, or which is a fragment of the sequence of amino acids 1-189 of SEQ ID NO: 10, that has protease activity; and   (d) a polypeptide having a sequence identity of at least 90% to the sequence of amino acids 1-189 of SEQ ID NO: 12, or which is a fragment of the sequence of amino acids 1-189 of SEQ ID NO: 12, that has protease activity.   
     
     
         22 . A nucleic acid construct comprising the nucleic acid sequence of  claim 21 , wherein the nucleic acid sequence is operably linked to one or more control sequences that direct the production of the polypeptide in a recombinant host cell. 
     
     
         23 . A recombinant expression vector comprising the nucleic acid construct of  claim 22 . 
     
     
         24 . A recombinant host cell comprising the nucleic acid construct of  claim 22 . 
     
     
         25 . A method for producing a polypeptide having protease activity, comprising
 (a) cultivating a recombinant host cell of  claim 24  under conditions for producing the polypeptide; and   (b) recovering the polypeptide.