US2016122778A1PendingUtilityA1

Stress tolerant plants and methods thereof

Assignee: MONSANTO TECHNOLOGY LLCPriority: Dec 10, 2003Filed: Oct 30, 2015Published: May 5, 2016

Est. expiryDec 10, 2023(expired)· nominal 20-yr term from priority

Inventors:Santanu Dasgupta Targolli L. Jayaprakash Carolyn J. Thai Kottaram Krishnadas Narayanan Thomas G. Ruff Stanton Dotson

C12N 15/8271C07K 14/415C12N 15/8273

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References

Claims

Abstract

The present invention provides a method and DNA molecules that when expressed in a plant produces transgenic plants with improved abiotic stress tolerance. The invention includes plant expression vectors comprising the DNA molecules, and plants containing such DNA molecules.

Claims

exact text as granted — not AI-modified

We claim: 
     
         1 . A method of generating a transgenic plant with enhanced tolerance to environmental stress comprising the steps of:
 a) transforming a plant cell with a DNA construct comprising a promoter that functions in plants, operably linked to a DNA polynucleotide molecule that encodes a protein substantially homologous to a protein selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51, and operably linked to a 3′ termination region; and   b) regenerating said plant cell into a fertile transgenic plant; and   c) selecting said fertile transgenic plant containing said DNA construct; wherein said fertile transgenic plant exhibits enhanced stress tolerance compared to a plant of a same plant species not transformed to contain said DNA construct.   
     
     
         2 . The method of  claim 1 , wherein said promoter is a plant virus promoter. 
     
     
         3 . The method of  claim 1 , wherein said promoter comprises a heterologus plant promoter. 
     
     
         4 . The method of  claim 1 , wherein said DNA molecule is substantially homologous to a DNA molecule selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46 and SEQ ID NO: 50. 
     
     
         5 . The method of  claim 1 , wherein said enhanced stress tolerance is cold stress tolerance. 
     
     
         6 . The method of  claim 1 , wherein said enhanced stress tolerance is water stress tolerance. 
     
     
         7 . The method of  claim 1 , wherein said crop plant cell is selected from the group consisting of corn, soybean, wheat, cotton, rice and rapeseed/canola. 
     
     
         8 . A transgenic plant with enhanced stress tolerance compared to a plant of a same plant species comprising a DNA construct, wherein said DNA construct comprises a promoter that functions in plants, operably linked to a DNA molecule that encodes a protein substantially homologous to a protein selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51 and operably linked to a 3′ termination region. 
     
     
         9 . The transgenic plant of  claim 8 , wherein said enhanced stress tolerance is cold tolerance. 
     
     
         10 . The transgenic plant of  claim 8 , wherein said enhanced stress tolerance is water stress tolerance. 
     
     
         11 . The progeny of said transgenic plant of  claim 8 . 
     
     
         12 . The transgenic plant of  claim 8  wherein said transgenic plant is selected from the group consisting of corn, soybean, wheat, cotton, rice and rapeseed/canola. 
     
     
         13 . A plant part produced by said transgenic plant of  claim 8  comprising leaves, roots, stems, shoot, flowers, fibers, fruit, or seed. 
     
     
         14 . A DNA construct, wherein said DNA construct comprises a promoter that functions in crop plants, operably linked to a DNA molecule that encodes a protein substantially homologous to a protein selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51, and operably linked to a 3′termination region. 
     
     
         15 . The DNA construct of  claim 14 , wherein said protein comprises at least a N-terminal substantial portion of a protein selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51. 
     
     
         16 . The DNA construct of  claim 14 , wherein said protein comprises at least a N-terminal 85% fragment of protein selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51. 
     
     
         17 . The DNA construct of  claim 14 , wherein said protein comprises at least a N-terminal 90% fragment of protein selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51. 
     
     
         18 . The DNA construct of  claim 14 , wherein said protein comprises at least a N-terminal 95% fragment of protein selected from the group consisting of: SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47 and SEQ ID NO: 51. 
     
     
         19 . The DNA construct of  claim 14 , wherein said DNA construct comprises a promoter that functions in crop plants, operably linked to a DNA molecule substantially homologous to DNA molecule selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 42, SEQ ID NO: 44, SEQ ID NO: 46 and SEQ ID NO: 50, and operably linked to a 3′termination region. 
     
     
         20 . A method of generating a transgenic crop plant with enhanced tolerance to cold stress comprising the steps of:
 a) transforming a plant cell with a DNA construct comprising a promoter that functions in plants, operably linked to a DNA molecule that encodes a protein substantially homologous to a protein selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, and SEQ ID NO: 51 and operably linked to a 3′termination region; and   b) regenerating said plant cell into a fertile transgenic crop plant; and   c) selecting said fertile transgenic crop plant containing said DNA construct;   wherein said crop plant exhibits enhanced cold stress tolerance compared to a plant of a same plant species not transformed to contain said DNA construct.

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