Novel metalloproteases

Abstract

Aspects of the present compositions and methods relate to novel metalloproteases, polynucleotides encoding the novel metalloproteases, and compositions and methods for use thereof.

1 . A polypeptide comprising an amino acid sequence having at least 60%, 80%, or 95% sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NOs: 3, 8, 13, 18, 23, 28, 33 and 38. 
     
     
         2 - 4 . (canceled) 
     
     
         5 . The polypeptide of  claim 1 , wherein said polypeptide is derived from a member of the order Bacillales or is derived from a  Planococcus  species. 
     
     
         6 . The polypeptide of  claim 5 , wherein said Bacillales member is a Paenibacillaceae family member or a  Paenibacillus  spp. 
     
     
         7 - 8 . (canceled) 
     
     
         9 . The polypeptide of  claim 1 , wherein said polypeptide has protease activity. 
     
     
         10 . The polypeptide of  claim 9 , wherein said protease activity comprises casein hydrolysis, collagen hydrolysis, elastin hydrolysis, keratin hydrolysis, soy protein hydrolysis or corn meal protein hydrolysis. 
     
     
         11 . The polypeptide of  claim 1 , wherein said polypeptide retains at least 50% of its maximal activity between pH 4.5 and 10 and/or between 30° C. and 70° C. 
     
     
         12 . (canceled) 
     
     
         13 . The polypeptide of  claim 1 , wherein said polypeptide has cleaning activity in a detergent composition. 
     
     
         14 . The polypeptide of  claim 13 , wherein said detergent composition is selected from an ADW detergent composition, a laundry detergent composition, a liquid laundry detergent composition, and a powder laundry detergent composition. 
     
     
         15 - 18 . (canceled) 
     
     
         19 . The polypeptide of  claim 1 , wherein said polypeptide is a recombinant polypeptide. 
     
     
         20 . A composition comprising the polypeptide of  claim 1 . 
     
     
         21 . The composition of  claim 20 , wherein said composition is a cleaning composition or a detergent composition. 
     
     
         22 . (canceled) 
     
     
         23 . The composition of  claim 21 , wherein said detergent composition is selected from the group consisting of a laundry detergent, a fabric softening detergent, a dishwashing detergent, and a hard-surface cleaning detergent. 
     
     
         24 . The composition of any of  claim 20 , wherein said composition further comprises a surfactant; at least one calcium ion and/or zinc ion; at least one stabilizer; from about 0.001 to about 0.1 weight % of said polypeptide; at least one bleaching agent; at least one adjunct ingredient; and/or one or more additional enzymes or enzyme derivatives selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1,4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, mannanases, oxidases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, and xylosidases, additional metallopotease enzymes and combinations thereof. 
     
     
         25 - 31 . (canceled) 
     
     
         32 . The composition of  claim 20 , wherein said cleaning composition contains phosphate or is phosphate-free. 
     
     
         33 - 34 . (canceled) 
     
     
         35 . The composition of  claim 20 , wherein said composition is a granular, powder, solid, bar, liquid, tablet, gel, or paste composition. 
     
     
         36 - 38 . (canceled) 
     
     
         39 . A method of cleaning, comprising contacting a surface or an item with a cleaning composition comprising the polypeptide of  claim 1 . 
     
     
         40 - 41 . (canceled) 
     
     
         42 . The method of  claim 39 , wherein said item is dishware or fabric. 
     
     
         43 - 48 . (canceled) 
     
     
         49 . A method for producing the polypeptide of  claim 1  comprising:
 a. stably transforming a host cell with an expression vector comprising a polynucleotide encoding the polypeptide of  claim 1 ; 
 b. cultivating said transformed host cell under conditions suitable for said host cell to produce said protease; and 
 c. recovering said protease. 
 
     
     
         50 - 56 . (canceled) 
     
     
         57 . A nucleic acid sequence comprising a nucleic acid sequence:
 (i) having at least 70% identity to a sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39, or   (ii) being capable of hybridizing to a probe derived from the polynucleotide sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39, under conditions of intermediate to high stringency, or   (iii) being complementary to the polynucleotide sequence selected from the group consisting of SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34 and 39.   
     
     
         58 . A vector comprising the nucleic acid sequence of  claim 57 . 
     
     
         59 . A host cell transformed with the vector of  claim 58 . 
     
     
         60 - 61 . (canceled) 
     
     
         62 . A textile processing, animal feed, leather processing, feather processing, or corn soy protein processing composition comprising the polypeptide of  claim 1 . 
     
     
         63 - 67 . (canceled)