Neutrophil-depleted whole blood and platelet rich plasma compositions

Abstract

A particle/cell separation device is described which is particularly adapted for neutrophil depletion from a preparation of whole blood or platelet-rich plasma. Also described are blood and platelet rich plasma compositions produced using the device which are neutrophil-depleted.

1 - 17 . (canceled) 
     
     
         18 . A cell separation device for producing neutrophil-depleted platelet-rich plasma with centrifugation, said device comprising:
 an inlet portion comprising an inlet port for receiving a sample of whole blood;   a separation chamber adapted to separate platelet-rich plasma from other components of the whole blood based on differences in specific gravity;   a flow path for the platelet-rich plasma comprising an area of constriction adapted to remove neutrophils from the platelet rich plasma; and   an outlet portion comprising a collection port in fluid communication with the separation chamber for removing neutrophil-depleted platelet-rich plasma.   
     
     
         19 . The cell separation device of  claim 18 , wherein the area of constriction of the flow path has cross-sectional dimension of 10-100 microns. 
     
     
         20 . The cell separation device of  claim 19 , wherein the area of constriction of the flow path has cross-sectional dimension of 10-30 microns. 
     
     
         21 . The cell separation device of  claim 18 , wherein the device produces platelet-rich plasma having a platelet concentration of between 2-8 times the platelet concentration in the whole blood. 
     
     
         22 . The cell separation device of  claim 18 , wherein the neutrophil concentration in the platelet-rich plasma is less than about 1000/μl. 
     
     
         23 . A kit for treating damaged tissue comprising:
 (i) a syringe for withdrawing blood from a patient;   (ii) a centrifuge device to obtain neutrophil-depleted platelet-rich plasma;   the centrifuge device comprising:
 an inlet portion comprising an inlet port for receiving a sample of blood; 
 a separation chamber adapted to separate platelet-rich plasma from other components of the blood based on differences in specific gravity; 
 a flow path for the platelet-rich plasma comprising an area of constriction adapted to remove neutrophils from the platelet rich plasma; and 
 an outlet portion comprising a collection port in fluid communication with the separation chamber for removing neutrophil-depleted platelet-rich plasma; and 
   (iii) a syringe for subcutaneous delivery of the neutrophil-depleted platelet-rich plasma to the damaged tissue.   
     
     
         24 . The kit of  claim 23 , wherein the kit does not contain an activator of the neutrophil-depleted platelet-rich plasma. 
     
     
         25 . The kit  claim 24 , wherein the syringe for subcutaneous delivery of the neutrophil-depleted platelet-rich plasma comprises a minimally invasive catheter or needle. 
     
     
         26 . The kit of  claim 23 , further comprising an activator of the neutrophil-depleted platelet-rich plasma. 
     
     
         27 . The kit of  claim 23 , further comprising a pH adjusting agent for the neutrophil-depleted platelet-rich plasma. 
     
     
         28 . The kit of  claim 23 , wherein the cell separation device produces platelet-rich plasma having a platelet concentration of between 2-8 times the platelet concentration in the whole blood. 
     
     
         29 . The kit of  claim 23 , wherein the neutrophil concentration in the platelet-rich plasma is less than about 1000/μl.