Method for speeding up plant growth and improving yield by altering expression levels of kinases and phosphatases

Abstract

Transgenic plants having increased growth rate and increase yield are disclosed, and methods for making the same. In one embodiment, the method comprises: transforming a plant or plant cell with a nucleic acid molecule comprising a plant kinase and/or phosphatase gene selected from NG6, NG21, NG24, NG28, and NG32, and over-expressing said kinase and/or phosphatase gene in the plant or plant cell.

1 . A method to make a transgenic plant having increased rate of plant growth and/or elevated plant yields comprising:
 a) introducing a gene expression construct into a plant or plant cell, wherein the construct comprises a nucleic acid molecule encoding a phosphatase or kinase and the nucleic acid molecule comprises:
 i) a sequence having at least 85% identity with SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 45, 47, 49, 93, 95, 97, 99, or 101; or 
 ii) a sequence encoding a polypeptide having at least 85% identity with SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 46, 48, 50, 94, 96, 98, 100, or 102; 
    wherein the sequence is operatively linked to upstream and downstream regulatory components comprising a heterologous promoter; and   b) overexpressing the nucleic acid molecule in the plant or plant cell, wherein the promoter drives the overexpression of the nucleic acid molecule.   
     
     
         2 . The method of  claim 1 , wherein the nucleic acid molecule comprises:
 i) a sequence selected from SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 45, 47, 49, 93, 95, 97, 99, or 101; or   ii) a sequence encoding a polypeptide selected from SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 46, 48, 50, 94, 96, 98, 100, or 102.   
     
     
         3 . The method of  claim 1 , further comprising regenerating, from said transformed plant or plant cell, a plant having enhanced growth and/or yield. 
     
     
         4 . The method of  claim 1 , wherein plant growth rate is increased. 
     
     
         5 . The method of  claim 1 , wherein plant yield is increased. 
     
     
         6 . The method of  claim 1 , wherein the plant is of a genus selected from the group consisting of:  Asparagus, Bromus, Hemerocallis, Hordeum, Lolium, Panicum, Pennisetum, Saccharum, Sorghum, Trigonella, Triticum, Zea, Antirrhinum, Arabidopsis, Arachis, Atropa, Brassica, Browallia, Capsicum, Carthamus, Cichorium, Citrus, Chrysanthemum, Cucumis, Datura, Daucus, Digitalis, Fragaria, Geranium, Glycine, Helianthus, Hyscyamus, Ipomoea, Latuca, Linum, Lotus, Majorana, Malva, Gossypium, Manihot, Medicago, Nemesia, Nicotiana, Onobrychis, Pelargonium, Petunia, Ranunculus, Raphanus, Salpiglossis, Senecio, Sinapis, Solanum, Trifolium, Vigna,  and  Vitis.    
     
     
         7 . The method of  claim 1 , wherein the plant is of a species of the genus  Brassica.    
     
     
         8 . The method of  claim 1 , wherein the plant is of a species selected from the group consisting of:  Glycine max, Zea mays, Solanum tuberosum, Panicum virgatum, Medicago sativa,  and  Brassica napus.    
     
     
         9 . The method of  claim 1 , wherein the plant cell is a seed, stem, shoot, or root cell. 
     
     
         10 . (canceled) 
     
     
         11 . The method of  claim 1 , wherein plant weight, total weight of leaf or seed, total number of inflorescence, carbon metabolism, level of carbohydrate, amino acid, lipid production or seed yield per plant are increased; or bolting time is earlier, when compared to a wild-type plant of the same species cultivated under the same conditions. 
     
     
         12 . A transgenic plant cell, comprising:
 a gene expression construct, wherein the construct comprises:
 i) a nucleic acid molecule comprising a sequence having at least 85% identity with SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 45, 47, 49, 93, 95, 97, 99, or 101, wherein said nucleic acid molecule is overexpressed in the transgenic plant cell when compared to plant cells of the same type in a wild-type plant of the same species; or 
 ii) a nucleic acid molecule that encodes a protein comprising a sequence having at least 85% identity with SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 11, 16, 48, 50, 94, 96, 98, 100, or 102, wherein said nucleic acid molecule is overexpressed in the transgenic plant cell when compared to plant cells of the same type in a wild-type plant of the same species; 
   wherein the sequence is operatively linked to upstream and downstream regulatory components comprising a heterologous promoter that drives the overexpression of the nucleic acid molecule in the plant cell.   
     
     
         13 . The transgenic plant cell of  claim 12 , comprising:
 i) a nucleic acid molecule comprising the sequence of SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 45, 47, 49, 93, 95, 97, 99, or 101, wherein said nucleic acid molecule is overexpressed in the transgenic plant cell when compared to plant cells of the same type in a wild-type plant of the same species; or   ii) a nucleic acid molecule that encodes a protein comprising the sequence of SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 94, 96, 98, 100, or 102.   
     
     
         14 . The transgenic plant cell of  claim 12 , wherein the plant cell comprises a nucleotide sequence having at least 85% identity with SEQ ID NO: 1, 3, 5, 7, 9. 
     
     
         15 . The transgenic plant cell of  claim 12 , wherein the plant cell comprises a nucleotide sequence encoding a sequence having at least 85% identity with SEQ ID NO:2, 4, 6, 8, or 10. 
     
     
         16 . The transgenic plant cell of  claim 12 , wherein said plant cell is of a monocotyledonous species. 
     
     
         17 . The transgenic plant cell of  claim 12 , wherein said plant cell is of a dicotyledonous species. 
     
     
         18 . The transgenic plant cell of  claim 12 , wherein the plant cell is of a genus selected from the group consisting of:  Asparagus, Bromus, Hemerocallis, Hordeum, Lolium, Panicum, Pennisetum, Saccharum, Sorghum, Trigonella, Triticum, Zea, Antirrhinum, Arabidopsis, Arachis, Atropa, Brassica, Browallia, Capsicum, Carthamus, Cichorium, Citrus, Chrysanthemum, Cucumis, Datura, Daucus, Digitalis, Fragaria, Geranium, Glycine, Helianthus, Hyscyamus, Ipomoea, Latuca, Linum, Lotus, Majorana, Malva, Gossypium, Manihot, Medicago, Nemesia, Nicotiana, Onobrychis, Pelargonium, Petunia, Ranunculus, Raphanus, Salpiglossis, Senecio, Sinapis, Solanum, Trifolium, Vigna,  and  Vitis.    
     
     
         19 . The transgenic plant cell of  claim 12 , wherein the plant cell is of a species of the genus  Brassica.    
     
     
         20 . A transgenic plant, comprising the transgenic plant cell of  claim 12 . 
     
     
         21 . The transgenic plant of  claim 20 , wherein plant weight, total weight of leaf or seed, total number of inflorescence, carbon metabolism, level of carbohydrate, amino acid, lipid production, or seed yield per plant are increased; or bolting time is earlier, when compared to a wild-type plant of the same species cultivated under the same conditions. 
     
     
         22 . The method of  claim 11 , wherein the seed yield per plant is increased by approximately 30% -50% as compared with a wild-type plant of the same species. 
     
     
         23 . The method of  claim 11 , wherein the bolting time per plant is earlier by approximately 19% as compared with a wild-type plant of the same species. 
     
     
         24 . The transgenic plant of  claim 21 , wherein the seed yield is increased by approximately 30%-50% as compared with a wild-type plant of the same species. 
     
     
         25 . The transgenic plant of  claim 21 , wherein the boiling time is earlier by approximately 19% as compared with a wild-type plant of the same species.