Kit of medium of inducing and amplifying hematopoietic stem cells

Abstract

A kit of medium of inducing and amplifying hematopoietic stem cells, the kit includes medium of inducing and amplifying hematopoietic stem cell, and the medium of inducing and amplifying hematopoietic stem cell includes cell proliferation factors and cell differentiation inhibitors needed in proliferation of hematopoietic stem cells, and the formula of the medium of inducing and amplifying hematopoietic stem cell is: 80-100% (v/v) DMEM/F12 basic medium, 0-1 μg/ml Sonic hedgehog, 0-1 μg/ml Delta1, 0-1 μg/ml Flt3L, 0-1 μg/ml IGFBP, 0-1 μg/ml SCF, 0-1 μg/ml, Angiopoietin, 0-1 μg/ml TPO, 0-1 μg/ml MBP, 0-1 μg/ml LIF, 0-1 μg/ml TGF-β, 0-100 mM MTEPA, and 1-5 mM NAC.

What is claimed is: 
     
         1 . A kit of medium of inducing and amplifying hematopoietic stem cells, characterized in that, the kit includes the medium of inducing and amplifying hematopoietic stem cell, and the medium of inducing and amplifying hematopoietic stem cell includes cell proliferation factors and cell differentiation inhibitors needed in proliferation of hematopoietic stem cells, and a formula of the medium of inducing and amplifying hematopoietic stem cell is: 80-100% (v/v) DMEM/F12 basic medium, 0-1 μg/ml Sonic hedgehog, 0-1 μg/ml Delta1, 0-1 μg/ml Flt3L, 0-1 μg/ml IGFBP, 0-1 μg/ml SCF, 0-1 μg/ml, Angiopoietin, 0-1 μg/ml TPO, 0-1 μg/ml MBP, 0-1 μg/ml LIF, 0-1 μg/ml TGF-β, 0-100 μM MTEPA, and 1-5 mM NAC.