Method for producing chimeric animal

Abstract

[Problem to be Solved] The present invention provides a method for producing a chimeric animal using a primed pluripotent stem cell, a tissue stem cell, a progenitor cell, a somatic cell, or a germ cell. [Solution] The method for producing a chimeric animal according to the present invention comprises introducing a mammal-derived cell into the embryo of a mammal, the cell being primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.

1 . A method for producing a chimeric animal, comprising
 introducing a mammal-derived cell to an embryo of a mammal, wherein the cell is a primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.   
     
     
         2 . The method according to  claim 1 , wherein the cell to be introduced to the embryo has been treated by cell death suppression treatment. 
     
     
         3 . The method according to  claim 1 , wherein the cell to be introduced to the embryo is a lineage-committed progenitor cell. 
     
     
         4 . The method according to  claim 3 , wherein the cell to be introduced to the embryo is an endodermal lineage progenitor cell. 
     
     
         5 . The method according to  claim 4 , wherein the cell to be introduced to the embryo is a Sox17-expressing cell. 
     
     
         6 . The method according to  claim 1 , wherein the cell to be introduced to the embryo is a pluripotent stem cell capable of forming a colony after being a dispersed single cell. 
     
     
         7 . A method for producing a chimeric animal, comprising
 introducing a human pluripotent stem cell to the embryo of a mammal,   wherein the cell has been subjected to cell death suppression treatment.   
     
     
         8 . A method for improving the ability of a mammal-derived cell to form a chimera, comprising
 subjecting the cell to cell death suppression treatment,   wherein the cell is a primed pluripotent stem cell, tissue stem cell, progenitor cell, somatic cell, or germ cell.   
     
     
         9 . A cell obtained by a method according to  claim 8 . 
     
     
         10 . A method for evaluating the differentiation potential of a cell or the in vivo function or safety of a cell, comprising
 producing a chimeric animal by a method according to  claim 1 , (provided that the cell to be evaluated is used as a cell that is introduced to the embryo of a nonhuman mammal) and   examining the contribution of the cell to each tissue in the produced chimeric animal to evaluate the differentiation potential of the cell into each tissue or the in vivo function or safety of the cell.   
     
     
         11 . A method for rendering a primed pluripotent stem cell less differentiated, comprising
 subjecting the primed pluripotent stem cell to cell death suppression treatment.   
     
     
         12 . An agent for use in promoting reprogramming of a primed pluripotent stem cell, comprising a cell death suppressor. 
     
     
         13 . A method for producing a naïve pluripotent stem cell, comprising
 subjecting a primed pluripotent stem cell to cell death suppression treatment. 
 
     
     
         14 . An agent for use in inducing a naïve pluripotent stem cell from a primed pluripotent stem cell, comprising a cell death suppressor.