Adenoviruses and their use

Abstract

Baboon Adenovirus (BaAdV)-2/4 and BaAdV-3 are disclosed herein. BaAdV-2/4 and BaAdV-3 polynucleotide, polypeptides and antibodies that specifically bind BaAdV-2/4 and/or BaAdV-3 are disclosed. Methods are disclosed for detecting BaAdV-2/4 and BaAdV-3. Methods are also disclosed for treating, preventing, and inducing an immune response to BaAdV-2/4 and/or BaAdV-3. Kits are also provided.

1 . An isolated recombinant nucleic acid molecule comprising a nucleic acid sequence at least 100 nucleotides in length that has at least 90% sequence identity over its length to SEQ ID NO:1, SEQ ID NO: 2, SEQ ID NO: 3, or the complement thereof, wherein the recombinant nucleic acid is:
 (a) a cDNA;   (b) attached to a detectable label; or   (c) operably linked to a heterologous nucleic acid.   
     
     
         2 . An isolated recombinant isolated nucleic acid molecule comprising:
 (a) a nucleic acid sequence at least 90% identical to nucleotides 1-29685 and 29867-34391 of the nucleic acid sequence set forth as SEQ ID NO: 1;   (b) a nucleic acid sequence at least 90% identical to nucleotides 1-29865 and 29867-34391 of the nucleic acid sequence set forth as SEQ ID NO: 2;   (c) a nucleotide sequence at least 90% identical to the nucleotide sequence set forth as nucleotides 1-28677 and 29812-34402 of SEQ ID NO: 3.   
     
     
         3 . The isolated recombinant nucleic acid molecule of  claim 2 , comprising the nucleic acid sequence set forth as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or a degenerate variant thereof. 
     
     
         4 . A cDNA comprising a nucleic acid sequence at least 90% identical to an open reading frame of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. 
     
     
         5 . The cDNA of  claim 4 , comprising an open reading frame of the nucleic acid sequence set forth as SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. 
     
     
         6 . An isolated polypeptide encoded by the nucleic acid sequence of  claim 4 . 
     
     
         7 . The isolated polypeptide of  claim 6 , comprising an amino acid sequence at least 90% identical to the amino acid sequence set forth as one of SEQ ID NOs: 5-109. 
     
     
         8 . An isolated polypeptide comprising the amino acid sequence encoded by the nucleotide sequence of  claim 5 . 
     
     
         9 . An isolated antibody that specifically binds to the polypeptide of  claim 6 . 
     
     
         10 . The isolated antibody of  claim 9 , wherein the antibody is a monoclonal antibody. 
     
     
         11 . The isolated antibody of  claim 9 , wherein the antibody is labeled. 
     
     
         12 . A composition comprising an effective amount of the nucleic acid of  claim 1 , a polypeptide encoded by the nucleic acid, a vector comprising the nucleic acid, a host cell comprising the vector, an adenovirus comprising the nucleic acid, molecule or antibody that specifically binds the polypeptide, and a pharmaceutically acceptable carrier. 
     
     
         13 . A method of detecting a Baboon adenovirus (BaAdV)-3 or a BaAdV-2/4 adenovirus, comprising
 (a) contacting a sample suspected of comprising an adenoviral nucleic acid with the nucleic acid of  claim 1  under stringent conditions; and   (b) detecting the presence or absence of hybridization of the nucleotide sequence to the sample, wherein the presence of hybridization detects the BaAdV-3 or a BaAdV-2/4 adenovirus.   
     
     
         14 . A method of detecting a Baboon adenovirus (BaAdV)-3 or BaAdV-2/4 nucleic acid, the method comprising the steps of:
 (a) contacting a sample suspected of comprising an adenoviral nucleic acid with at least one primer that hybridizes under stringent conditions to the nucleotide sequence set forth as SEQ ID NO:1, SEQ ID NO: 2, and/or SEQ ID NO: 3;   (b) performing a PCR reaction to amplify cDNA; and   (c) detecting presence or absence of the cDNA, wherein the presence of the cDNA detects the BaAdV-3 or a BaAdV-2/4 adenovirus.   
     
     
         15 . A method of detecting a baboon adenovirus BaAdV-3 or a BaAdV-2/4 infection in a subject, comprising:
 (a) contacting a sample from the subject suspected of having an infection caused by the BaAdV-3 or the BaAdV-2/4 with the antibody of  claim 9 ; and   (b) detecting binding of the antibody to a polypeptide in the sample, wherein binding of the antibody to the polypeptide detects the presence of the BaAdV-3 or the BaAdV-2/4 infection.   
     
     
         16 . A kit, comprising
 a container comprising the isolated recombinant nucleic acid molecule of  claim 1 , a polypeptide encoded by the nucleic acid or an immunogenic fragment of the polypeptide, a vector comprising the nucleic acid, a host cell comprising the vector, an adenovirus comprising the nucleic acid, or an antibody that specifically binds the polypeptide, or a primer that hybridizes to the nucleotide sequence set forth SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3 under highly stringent conditions; and   instructions for using the kit.   
     
     
         17 . A kit, comprising
 the polypeptide of  claim 6 , and   instructions for the detection of the presence of antibodies that specifically bind Baboon adenovirus (BaAdV)-3 or BaAdV-4 in a sample from a subject.   
     
     
         18 . A kit comprising
 the antibody of any  claim 9 ; and   instructions for the detection of the presence of a Baboon adenovirus (BaAdV)-3 or BaAdV-4 polypeptide in a sample from a subject.   
     
     
         19 . An isolated polypeptide comprising the amino acid sequence set forth as one of SEQ ID NOs: 5-109. 
     
     
         20 . The isolated polypeptide of  claim 20 , consisting of the amino acid sequence set forth as one of SEQ ID NOs: 5-109. 
     
     
         21 . An adenovirus comprising the polypeptide of  claim 20 . 
     
     
         22 . The method of  claim 15 , wherein the antibody specifically binds the amino acid sequence set forth as one of SEQ ID NOs: 5-109.