Polypeptide variants with altered effector function

Abstract

The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.

1 - 25 . (canceled) 
     
     
         26 . Isolated nucleic acid encoding an antibody, wherein the antibody comprises a variant IgG1 Fc region which is not a native sequence Fc region, wherein the variant IgG1 Fc region comprises an amino acid substitution at amino acid position 239, and wherein the numbering of the residues in the Fc region is that of the EU index as in Kabat. 
     
     
         27 . A vector comprising the nucleic acid of  claim 26 . 
     
     
         28 . A host cell comprising the vector of  claim 27 . 
     
     
         29 . The nucleic acid of  claim 26 , where the IgG1 Fc region is a human Fc region. 
     
     
         30 . A method for producing an antibody comprising a variant IgG1 Fc region comprising an amino acid substitution at position 239 of the Fc region, wherein the numbering is that of the EU index as in Kabat, wherein the method comprises culturing the host cell of  claim 28  so that the nucleic acid is expressed. 
     
     
         31 . The method of  claim 30 , further comprising recovering the antibody from the host cell culture. 
     
     
         32 . The method of  claim 30 , wherein the variant IgG1 Fc region is a human Fc region.