Methods and systems for differential extraction

Abstract

Methods are provided for differential extraction of DNA from at least two different cell types. Systems for carrying out the differential extraction methods are also provided. A kit is also provided for differential extraction of DNA from at least two different cell types using a multi-compartment container.

I claim: 
     
         1 . A system for differential extraction of sperm cells, comprising a plurality of compartments, wherein at least one compartment comprises a selective sperm lysis buffer. 
     
     
         2 . The system of  claim 1 , comprising a first compartment comprising a cell-trapping matrix and a second compartment comprising a selective sperm lysis buffer. 
     
     
         3 . The system of  claim 2 , further comprising a third. compartment comprising a plurality of DNA-binding particles. 
     
     
         4 . The system of  claim 3 , further comprising a fourth compartment comprising an elution buffer. 
     
     
         5 . The system of  claim 1  comprising: a first compartment comprising a cell-trapping matrix; a second compartment comprising a cell wash buffer; a third compartment comprising a selective sperm lysis buffer; a fourth compartment comprising a plurality of DNA binding particles; a fifth compartment comprising a DNA wash buffer; and a sixth compartment. comprising an elution buffer. 
     
     
         6 . The system of  claim 5 , wherein the first, second, third, fourth, fifth, and sixth compartments are part of a removable cartridge. 
     
     
         7 . The system of  claim 5 , further comprising a seventh compartment comprising a DNA-binding buffer.  
     
     
         8 . The system of  claim 7 , wherein the seventh compartment further comprises a second plurality of DNA-binding particles. 
     
     
         9 . The system of  claim 7 , further comprising an eighth compartment comprising a second plurality of DNA-binding particles. 
     
     
         10 .- 27 . (canceled)