Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods

Abstract

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering metabolism in a cell using isolated and/or purified polypeptides and nucleic acid sequences from Alicyclobacillus acidocaldarius.

What is claimed is: 
     
         1 . An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID No. 392. 
     
     
         2 . The expression vector of  claim 1 , wherein the expression vector comprises a polynucleotide having at least 90% identity to SEQ ID No. 393. 
     
     
         3 . The expression vector of  claim 1 , wherein the encoded polypeptide has Acetyl-CoA C-acetyltransferase enzymatic activity. 
     
     
         4 . A method of modulating or altering metabolism in a cell, the method comprising:
 providing the expression vector of  claim 3  to the cell and expressing the encoded polypeptide in the cell.   
     
     
         5 . The method according to  claim 4 , further comprising glycosylating, or otherwise post-translationally modifying the encoded peptide in the cell. 
     
     
         6 . An expression vector comprising an isolated polynucleotide encoding a polypeptide having at least 95% sequence identity to SEQ ID No. 392. 
     
     
         7 . The expression vector of  claim 6 , wherein the encoded polypeptide has Acetyl-CoA C-acetyltransferase enzymatic activity. 
     
     
         8 . The expression vector of  claim 6 , wherein the expression vector comprises a polynucleotide having at least 95% identity to SEQ ID No. 393.