US2016046897A1PendingUtilityA1

Organ mimic device with microchannels and methods of use and manufacturing thereof

66
Assignee: CHILDRENS MEDICAL CENTERPriority: Jul 16, 2008Filed: Oct 26, 2015Published: Feb 18, 2016
Est. expiryJul 16, 2028(~2 yrs left)· nominal 20-yr term from priority
Inventors:Donald E. IngberDongeun Huh
C12N 5/0696C12N 5/0654B01L 2400/0481B01L 3/50273C12M 25/02B01L 2300/0877G01N 33/5088C12N 5/0671C12M 29/10B01L 2200/0663B01L 2300/163B01L 2300/0854B01L 3/5027C12N 5/0688B01L 2400/0487C12M 21/08C12M 23/16C12N 5/0623B01L 2300/0887C12N 5/0647G01N 33/5091C12N 5/061B01L 2400/0472G01N 33/5005C12N 5/0662C12M 23/58C12N 5/0697C12N 5/0606C12M 29/00C12M 35/04
66
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Claims

Abstract

System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A method, comprising: a) providing a microfluidic device having a body, the body including a fluid channel and a membrane, the fluid channel containing stem cells; and b) applying fluid to said stem cells through said fluid channel. 
     
     
         2 . The method of  claim 1 , wherein said membrane is positioned along a plane within said fluid channel to partition the channel into a first microchannel and a second microchannel. 
     
     
         3 . The method of  claim 1 , further comprising c) allowing differentiation cues to reach the stem cells thereby differentiating the stem cells to different cell types. 
     
     
         4 . The method of  claim 1 , wherein said stem cells are human embryonic stem cells. 
     
     
         5 . The method of  claim 1 , wherein said stem cells are induced pluripotent stem cells. 
     
     
         6 . The method of  claim 1 , wherein said stem cells are stem cells isolated from adult tissues. 
     
     
         7 . The method of  claim 1 , wherein said stem cells are mesenchymal stem cells. 
     
     
         8 . The method of  claim 1 , wherein said stem cells are hematopoietic stem cells. 
     
     
         9 . The method of  claim 1 , wherein said stem cells are stem cell of osteoblasts. 
     
     
         10 . The method of  claim 1 , wherein said stem cells are stem cell for neurons. 
     
     
         11 . The method of  claim 1 , wherein said stem cells are stem cell for olfactory neurons. 
     
     
         12 . The method of  claim 1 , wherein said stem cells are stem cell for spermatocytes. 
     
     
         13 . The method of  claim 1 , wherein said culture fluid is serum free. 
     
     
         14 . The method of  claim 1 , wherein said method further comprises c) contacting said cells with an agent and d) measuring the response of said cells to said agent. 
     
     
         15 . The method of  claim 14 , wherein said agent is a chemical agent. 
     
     
         16 . The method of  claim 14 , wherein said agent is a biological agent. 
     
     
         17 . The method of  claim 14 , wherein said agent is a pharmacological agent. 
     
     
         18 . A microfluidic device comprising a body, the body including a fluid channel and a membrane, the fluid channel containing stem cells in fluid. 
     
     
         19 . The microfluidic device of  claim 18 , wherein said membrane is positioned along a plane within said fluid channel to partition the channel into a first microchannel and a second microchannel. 
     
     
         20 . The microfluidic device of  claim 18 , wherein said stem cells are human embryonic stem cells. 
     
     
         21 . The microfluidic device of  claim 18 , wherein said stem cells are induced pluripotent stem cells. 
     
     
         22 . The microfluidic device of  claim 18 , wherein said stem cells are stem cells isolated from adult tissues. 
     
     
         23 . The microfluidic device of  claim 18 , wherein said stem cells are mesenchymal stem cells. 
     
     
         24 . The microfluidic device of  claim 18 , wherein said stem cells are hematopoietic stem cells. 
     
     
         25 . The microfluidic device of  claim 18 , wherein said stem cells are stem cell of osteoblasts. 
     
     
         26 . The microfluidic device of  claim 18 , wherein said stem cells are stem cell for neurons. 
     
     
         27 . The microfluidic device of  claim 18 , wherein said stem cells are stem cell for olfactory neurons. 
     
     
         28 . The microfluidic device of  claim 18 , wherein said stem cells are stem cell for spermatocytes.

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