Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . An isolated polypeptide having xylanase activity, selected from the group consisting of:
 (a) a polypeptide having at least 67%, e.g., at least 68%, at least 69%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 2, a polypeptide having at least 61%, e.g., at least 62%, at least 63%, at least 65%, at least 67%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 4, a polypeptide having at least 61%, e.g., at least 62%, at least 63%, at least 65%, at least 67%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 6, a polypeptide having at least 90%, e.g., at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 8, a polypeptide having at least 79%, e.g., at least 80%, at least 83%, at least 85%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 10, or a polypeptide having at least 92%, e.g., at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO: 12;   (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, or medium stringency conditions, or medium-high stringency conditions, or high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, or SEQ ID NO: 11, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);   (c) a polypeptide encoded by a polynucleotide having at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, or SEQ ID NO: 11; or the cDNA sequence thereof;   (d) a variant of the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 12 comprising a substitution, deletion, and/or insertion at one or more positions; and   (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has xylanase activity.   
     
     
         2 . (canceled) 
     
     
         3 . (canceled) 
     
     
         4 . (canceled) 
     
     
         5 . The polypeptide of  claim 1 , comprising or consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 12 or the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, or SEQ ID NO: 12. 
     
     
         6 . (canceled) 
     
     
         7 . (canceled) 
     
     
         8 . (canceled) 
     
     
         9 . (canceled) 
     
     
         10 . (canceled) 
     
     
         11 . (canceled) 
     
     
         12 . (canceled) 
     
     
         13 . (canceled) 
     
     
         14 . (canceled) 
     
     
         15 . (canceled) 
     
     
         16 . A nucleic acid construct or expression vector comprising a polynucleotide encoding the polypeptide of  claim 1  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         17 . A recombinant host cell comprising the polynucleotide nucleic acid construct or expression vector of  claim 16 . 
     
     
         18 . (canceled) 
     
     
         19 . A method of producing a polypeptide having xylanase activity, comprising:
 (a) cultivating the host cell of  claim 17  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         20 . A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         21 . A method of producing a polypeptide having xylanase activity, comprising:
 (a) cultivating the transgenic plant or plant cell of  claim 20  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         22 . (canceled) 
     
     
         23 . (canceled) 
     
     
         24 . (canceled) 
     
     
         25 . (canceled) 
     
     
         26 . A process for degrading a cellulosic material or xylan-containing material, comprising: treating the cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having xylanase activity of  claim 1 . 
     
     
         27 . The process of  claim 26 , wherein the cellulosic material or xylan-containing material is pretreated. 
     
     
         28 . The process of  claim 26 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin. 
     
     
         29 . (canceled) 
     
     
         30 . (canceled) 
     
     
         31 . The process of  claim 26 , further comprising recovering the degraded or converted cellulosic material or xylan-containing material. 
     
     
         32 . The process of  claim 31 , wherein the degraded cellulosic material or xylan-containing material is a sugar. 
     
     
         33 . (canceled) 
     
     
         34 . A process for producing a fermentation product, comprising:
 (a) saccharifying a cellulosic material or xylan-containing material with an enzyme composition in the presence of the polypeptide having xylanase activity of  claim 1 ;   (b) fermenting the saccharified cellulosic material or xylan-containing material with one or more fermenting microorganisms to produce the fermentation product; and   (c) recovering the fermentation product from the fermentation.   
     
     
         35 . The process of  claim 34 , wherein the cellulosic material or xylan-containing material is pretreated. 
     
     
         36 . The process of  claim 34 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin. 
     
     
         37 . (canceled) 
     
     
         38 . (canceled) 
     
     
         39 . The process of  claim 34 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation. 
     
     
         40 . (canceled) 
     
     
         41 . A process of fermenting a cellulosic material or xylan-containing material, comprising: fermenting the cellulosic material or xylan-containing material with one or more fermenting microorganisms, wherein the cellulosic material or xylan-containing material is saccharified with an enzyme composition in the presence of the polypeptide having xylanase activity of  claim 1 . 
     
     
         42 . The process of  claim 41 , wherein the fermenting of the cellulosic material or xylan-containing material produces a fermentation product and optionally further comprising recovering the fermentation product from the fermentation. 
     
     
         43 . (canceled) 
     
     
         44 . The process of  claim 1 , wherein the cellulosic material or xylan-containing material is pretreated before saccharification. 
     
     
         45 . The process of  claim 41 , wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin. 
     
     
         46 - 48 . (canceled)