Oncolytic poliovirus for human tumors

Abstract

Human clinical use of a chimeric poliovirus construct has demonstrated excellent anti-tumor effect. The mechanism of action is believed to involve both viral oncolysis as well as immune recruitment, both of which lead to necrosis in the area of the tumor. No adverse effects have been observed.

1 . A method of treating a human harboring a solid tumor which expresses NECL5 (nectin-like protein 5), said method comprising the steps of:
 administering directly to the tumor in the human a chimeric poliovirus construct comprising a Sabin type I strain of poliovirus with a human rhinovirus 2 (HRV2) internal ribosome entry site (IRES) in said poliovirus' 5′ untranslated region between said poliovirus' cloverleaf and said poliovirus' open reading frame.   
     
     
         2 . The method of  claim 1  wherein convection enhanced delivery is used to administer the chimeric poliovirus construct. 
     
     
         3 . The method of  claim 1  wherein the solid tumor is a glioblastoma. 
     
     
         4 . The method of  claim 1  wherein the solid tumor is a prostate tumor. 
     
     
         5 . The method of  claim 1  wherein the administration is intracerebral. 
     
     
         6 . The method of  claim 1  wherein the solid tumor is a medulloblastoma. 
     
     
         7 . The method of  claim 1  wherein the solid tumor is a breast tumor. 
     
     
         8 . The method of  claim 1  wherein the solid tumor is a lung tumor. 
     
     
         9 . The method of  claim 1  wherein the solid tumor is a colorectal tumor. 
     
     
         10 . The method of  claim 1  wherein the administration is intracerebral infusion with convection enhanced delivery. 
     
     
         11 . The method of  claim 10  wherein the administration is stereotactically guided. 
     
     
         12 . The method of  claim 1  wherein the human is an adult. 
     
     
         13 . The method of  claim 1  wherein the human is a child. 
     
     
         14 . The method of  claim 12  wherein concurrent or serial chemotherapy is administered to the human. 
     
     
         15 . The method of  claim 13  wherein concurrent or serial radiotherapy is administered to the human. 
     
     
         16 . The method of  claim 1  wherein the solid tumor is subjected to surgical removal before or after administering the nonpathogenic, oncolytic, poliovirus. 
     
     
         17 . The method of  claim 1  wherein prior to the step of administering the solid tumor is tested for expression of NECL5. 
     
     
         18 . A method of treating a human harboring a solid tumor which expresses NECL5 (nectin-like protein 5), said method comprising the steps of:
 testing a solid tumor to ascertain that it expresses NECL5;   administering directly to the tumor in the human a chimeric poliovirus construct comprising a Sabin type I strain of poliovirus with a human rhinovirus 2 (HRV2) internal ribosome entry site (IRES) in said poliovirus' 5′ untranslated region between said poliovirus' cloverleaf and said poliovirus' open reading frame, wherein the administering is stereotactically guided, intracerebral infusion with convection enhanced delivery.   
     
     
         19 . A clinical pharmaceutical preparation of a chimeric poliovirus construct, comprising:
 a Sabin type I strain of poliovirus with a human rhinovirus 2 (HRV2) internal ribosome entry site (IRES) in said poliovirus' 5′ untranslated region between said poliovirus' cloverleaf and said poliovirus' open reading frame; and   gadolinium.   
     
     
         20 . The clinical pharmaceutical preparation of  claim 19  wherein the gadolinium is chelated with diethylene triamine pentaacetic acid (DPTA). 
     
     
         21 . The clinical pharmaceutical preparation of  claim 19  further comprising human serum albumin. 
     
     
         22 . The clinical pharmaceutical preparation of  claim 21  wherein the human serum albumin is radiolabeled. 
     
     
         23 . The clinical pharmaceutical preparation of  claim 22  wherein the human serum albumin is radiolabeled with  124 I. 
     
     
         24 . A method of delivering a clinical pharmaceutical preparation to a solid tumor in a human, comprising:
 administering via convection enhanced infusion through a single intratumoral catheter, a dose of a poliovirus construct within 6.5 hours, wherein said poliovirus construct is in a clinical pharmaceutical preparation comprising a Sabin type I strain of poliovirus with a human rhinovirus 2 (HRV2) internal ribosome entry site (IRES) in said poliovirus' 5′ untranslated region between said poliovirus' cloverleaf and said poliovirus' open reading frame; and gadolinium.   
     
     
         25 . The method of  claim 24  wherein the tumor is a brain tumor. 
     
     
         26 . The method of  claim 24  wherein the tumor is a glioblastoma multiforme. 
     
     
         27 . The method of  claim 24  wherein the tumor is a pancreatic tumor. 
     
     
         28 . The method of  claim 24  wherein the tumor is a prostate tumor. 
     
     
         29 . The method of  claim 24  wherein the dose is delivered over a 6 hour period. 
     
     
         30 . The method of  claim 24  wherein the tumor expresses NECL5. 
     
     
         31 . The method of  claim 24  further comprising the step of testing the solid tumor to ascertain that it expresses NECL5. 
     
     
         32 . The method of  claim 1  wherein the chimeric poliovirus construct is in a clinical pharmaceutical preparation comprising gadolinium. 
     
     
         33 . The method of  claim 18  wherein the chimeric poliovirus construct is in a clinical pharmaceutical preparation comprising gadolinium. 
     
     
         34 . The method of  claim 1  wherein the method further comprises administering a biological therapy. 
     
     
         35 . The method of  claim 18  wherein the method further comprises administering a biological therapy. 
     
     
         36 . The method of  claim 24  wherein the method further comprises administering a biological therapy.