Compositions and methods for improving resolution of biomolecules separated on polyacrylamide gels

Abstract

Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

1 - 20 . (canceled) 
     
     
         21 . A cassette for performing native gel electrophoresis, wherein the cassette has a consistent gap width across its upper edge. 
     
     
         22 . The cassette of  claim 21 , wherein the cassette has a gap width across its upper edge in the range from 0.1 millimeters to 5 millimeters. 
     
     
         23 . The cassette of  claim 21 , wherein the gap width varies by less than 5%. 
     
     
         24 . The cassette of  claim 23 , wherein the variation in the gap width is 2% or less. 
     
     
         25 . The cassette of  claim 23 , wherein the cassette contains a gel that comprises polyacrylamide. 
     
     
         26 . The cassette of  claim 25 , wherein the gel is a non-denaturing gel. 
     
     
         27 . The cassette of  claim 26 , wherein the gel is a blue native gel. 
     
     
         28 . The cassette of  claim 26 , wherein the gel is a gradient gel. 
     
     
         29 . The cassette of  claim 21 , wherein the cassette comprises plastic. 
     
     
         30 . The cassette of  claim 29 , wherein the cassette is made by welding a front plate to a back plate. 
     
     
         31 - 41 . (canceled)