Multilayer fluidic devices and methods for their fabrication

Abstract

A fluidic device including an inorganic solid support attached to an organic solid support by a bonding layer, wherein the inorganic solid support has a rigid structure and wherein the bonding layer includes a material that absorbs radiation at a wavelength that is transmitted by the inorganic solid support or the organic solid support; and a channel formed by the inorganic solid support and the organic solid support, wherein the bonding layer that attaches the inorganic solid support to the organic solid support provides a seal against liquid flow. Methods for making fluidic devices, such as this, are also provided.

1 . A method for making a multilayer device, comprising
 (a) providing an inorganic solid support and an organic solid support, wherein the inorganic solid support comprises a rigid structure;   (b) providing a radiation-absorbing material, wherein the radiation-absorbing material is not a metal;   (c) contacting the inorganic solid support, the organic solid support and the radiation-absorbing material in a configuration wherein the radiation-absorbing material is present at an interface between the inorganic solid support and the organic solid support; and   (d) applying compression at the interface and irradiating the radiation-absorbing material with the radiation to form a bonding layer between the inorganic solid support and the organic solid support.   
     
     
         2 . The method of  claim 1 , wherein the multilayer device comprises a fluidic device having a channel formed by attachment of the inorganic solid support to the organic solid support. 
     
     
         3 . (canceled) 
     
     
         4 . (canceled) 
     
     
         5 . The method of  claim 2 , wherein the creating of the chemically reactive layer comprises silanizing the surface of the inorganic layer. 
     
     
         6 - 8 . (canceled) 
     
     
         9 . The method of  claim 1 , wherein the radiation passes through the inorganic layer or through the organic layer during the irradiating of the radiation-absorbing layer. 
     
     
         10 . The method of  claim 1 , wherein the irradiating forms covalent bonds between the inorganic solid support and the organic solid support. 
     
     
         11 . (canceled) 
     
     
         12 . The method of  claim 1 , wherein the organic solid support has a rigid structure and the irradiating softens the inorganic support or the organic solid support to create contact at the interface. 
     
     
         13 . (canceled) 
     
     
         14 . The method of  claim 1 , wherein the organic solid support comprises a thermoplastic and wherein the inorganic solid support comprises glass. 
     
     
         15 . The method of  claim 1 , wherein the radiation-absorbing material comprises a dye or carbon black. 
     
     
         16 . The method of  claim 1 , wherein the inorganic solid support comprises indium tin oxide. 
     
     
         17 . (canceled) 
     
     
         18 . The method of  claim 1 , further comprising
 (e) providing a second inorganic solid support;   (f) contacting the second inorganic solid support with the organic solid support in a configuration wherein the radiation-absorbing material is present at a second interface between the second inorganic solid support and the organic solid support; and   (g) applying compression at the second interface and irradiating the radiation-absorbing material with the radiation to form a bonding layer between the second inorganic solid support and the organic solid support.   
     
     
         19 . (canceled) 
     
     
         20 . (canceled) 
     
     
         21 . A fluidic device comprising
 (a) an inorganic solid support attached to an organic solid support by a bonding layer, wherein the inorganic solid support has a rigid structure and wherein the bonding layer comprises a material that absorbs radiation at a wavelength that is transmitted by the inorganic solid support or the organic solid support, wherein the material that absorbs the radiation is not a metal, and   (b) at least one channel formed by the attachment of the inorganic solid support to the organic solid support, wherein the channel is configured to contain a liquid and the bonding layer that attaches the inorganic solid support to the organic solid support provides a seal against liquid flow.   
     
     
         22 . The fluidic device of  claim 21 , wherein the organic solid support has a rigid structure. 
     
     
         23 . The fluidic device of  claim 21 , wherein the organic solid support comprises a thermoplastic. 
     
     
         24 . The fluidic device of  claim 21 , wherein the inorganic solid support comprises glass. 
     
     
         25 . (canceled) 
     
     
         26 . The fluidic device of  claim 21 , wherein the material that absorbs radiation comprises a dye or carbon black. 
     
     
         27 . The fluidic device of  claim 21 , further comprising a conducting layer between the inorganic solid support and the material that absorbs the radiation. 
     
     
         28 . (canceled) 
     
     
         29 . (canceled) 
     
     
         30 . The fluidic device of  claim 21 , wherein the organic solid support is further attached to a second inorganic solid support, whereby the organic solid support is sandwiched between the inorganic solid support and the second inorganic solid support. 
     
     
         31 . The fluidic device of  claim 30 , wherein the second inorganic solid support is attached to the organic solid support by a second bonding layer that comprises the material that absorbs radiation at the wavelength that is transmitted by the inorganic solid support or the organic solid support. 
     
     
         32 . (canceled) 
     
     
         33 . (canceled) 
     
     
         34 . The fluidic device of  claim 21 , further comprising an array of nucleic acid features attached to the inorganic solid support in the at least one channel. 
     
     
         35 - 37 . (canceled) 
     
     
         38 . A fluidic device comprising at least one channel defined by an inorganic solid support attached to an organic solid support by a bonding layer, wherein the fluidic device is made by the process of:
 (a) contacting the inorganic solid support, the organic solid support and a radiation-absorbing material in a configuration wherein the radiation-absorbing material is present at an interface between the inorganic solid support and the organic solid support, wherein the radiation-absorbing material is not a metal; and   (b) applying compression at the interface and irradiating the radiation-absorbing material with the radiation to form the bonding layer between the inorganic solid support and the organic solid support.