Dielectrophoretic cell capture

Abstract

Various aspects are described for selectivity capturing cells or bioparticles on designated surfaces in dielectrophoretic systems and processes. A particular adhesive composition is described for enhancing cell retention. In addition, certain permeable polyester membranes used in the systems and processes are also described.

1 . A layered composition for capturing cells and bioparticles during dielectrophoresis (DEP), the layered composition comprising:
 at least one layer of an adhesion material; and   a layer of a polycation material disposed on at least one layer of the adhesion material, the layer of the polycation material providing an exposed face for capturing cells and bioparticles during dielectrophoresis.   
     
     
         2 . The layered composition of  claim 1  wherein the polycation material is selected from the group consisting of poly(allylamine hydrochloride) (PAH), poly(ethylene imine) (PEI), poly (diallyl-dimethyl ammonium) chloride (PDADMAC), poly(lysine), polyacrylamide (PAAm), and combinations thereof. 
     
     
         3 . The layered composition of  claim 2  wherein the polycation material is poly(allylamine hydrochloride) (PAH). 
     
     
         4 . The layered composition of  claim 1  wherein the adhesion material is selected from the group consisting of fibronectin (FN), laminin, elastin, collagen, collagen fibrils, proteoglycans, hyaluronic acid, an extracellular matrix material analogue, and combination thereof. 
     
     
         5 . The layered composition of  claim 1  wherein the layer of the polycation material is adsorbed on the layer of the adhesion material. 
     
     
         6 .- 15 . (canceled)