Arrays, substrates, devices, methods and systems for detecting target molecules

Abstract

Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

1 . An array for detecting at least one target in a sample, the array comprising:
 at least one capture agent or component thereof attached to a substrate, the at least one capture agent capable of specifically binding the at least one target to form a capture agent target binding complex,   the at least one capture agent or component thereof arranged on the array so that capture agent target binding complexes are detectable along substantially parallel lines forming a barcoded pattern.   
     
     
         2 . The array of  claim 1 , wherein the at least one target is a plurality of targets, and the at least one capture agent or component thereof is a plurality of capture agents or components thereof, each capture agent of the plurality of capture agents bindingly distinguishable and positionally distinguishable from another, each capture agent of the plurality of capture agents capable of specifically binding each target of the plurality of targets to form a capture agent target binding complex. 
     
     
         3 - 5 . (canceled) 
     
     
         6 . The array of  claim 1 , wherein said substantially parallel lines are formed by microfluidic channels or portions thereof, the microfluidic channels being microfluidic channels of the array. 
     
     
         7 - 8 . (canceled) 
     
     
         9 . A microfluidic device comprising the array of  claim 1 . 
     
     
         10 . The microfluidic device of  claim 9 , further comprising a separating unit for separating a fluidic component of a fluid sample, the separating unit comprising
 a flowing microfluidic channel in fluidic communication with the inlet, the flowing microfluidic channel having a flowing channel resistance, and   an assaying microfluidic channel in fluidic communication with the flowing channel, the assaying microfluidic channel having an assaying channel resistance,   wherein the flowing microfluidic channel resistance and the assaying microfluidic channel resistance are adapted to control flowing of the fluidic component from the flowing microfluidic channel to the assaying microfluidic channel and   wherein the array is located on the assaying microfluidic.   
     
     
         11 . The microfluidic device of  claim 9 , wherein the at least one target is a plurality of targets, and the at least one capture agent or component thereof is a plurality of capture agents or components thereof, each capture agent of the plurality of capture agents bindingly distinguishable and positionally distinguishable from another, each capture agent of the plurality of capture agents capable of specifically binding each target of the plurality of targets to form a capture agent target binding complex. 
     
     
         12 - 13 . (canceled) 
     
     
         14 . A system for the detection of at least one target in a sample, the system comprising
 the array of  claim 1 ; and   a device for detecting the barcoded pattern on the array.   
     
     
         15 . The system of  claim 14 , wherein the at least one target is a plurality of targets, and the at least one capture agent or component thereof is a plurality of capture agents or components thereof, each capture agent of the plurality of capture agents bindingly distinguishable and positionally distinguishable from another, each capture agent of the plurality of capture agents capable of specifically binding each target of the plurality of targets to form a capture agent target binding complex. 
     
     
         16 - 19 . (canceled) 
     
     
         20 . A method for detecting a plurality of targets in a sample, the method comprising
 contacting said sample with the array of  claim 2 , for a time and under conditions to allow binding of said plurality of targets with said plurality of capture agents to form capture agent target binding complexes; and   detecting said capture agent target binding complexes.   
     
     
         21 . A substrate for detecting at least one detectable target, the substrate configured to allow attachment of said at least one detectable target on the substrate along substantially parallel lines, the substantially parallel lines forming a barcoded pattern. 
     
     
         22 . A microfluidic device comprising the substrate according to  claim 21 . 
     
     
         23 . A system for detecting a plurality of detectable targets, the system comprising: the substrate according to  claim 21  and a device for detecting the barcoded pattern. 
     
     
         24 . A method for detecting a plurality of targets, in a sample, the method comprising: contacting said sample with the substrate according to  claim 21  for a time and under conditions to allow binding of said plurality of targets with said substrate; and detecting said plurality of targets attached to the substrate. 
     
     
         25 . A method to attach a molecule on a fluidic support along a predetermined microfluidic pattern, the method comprising:
 providing a mold, the mold comprising fluidic channels, each microfluidic channel having an inlet and an outlet, each of the outlets of the channels configured to form part of the predetermined pattern,   providing the support, said support suitable to be coupled with the mold,   coupling the mold with the support,   providing the molecule in the fluidic channels for a time and under conditions to allow attachment of the molecule on the support; and   decoupling the mold from the support.   
     
     
         26 . The method of  claim 25 , wherein said pattern comprises substantially parallel lines forming a barcoded pattern. 
     
     
         27 - 28 . (canceled) 
     
     
         29 . The method according to  claim 25 , wherein the fluidic support is a microfluidic support and the fluidic channels are microfluidic channels. 
     
     
         30 . A system to attach a molecule on a fluidic support along a predetermined microfluidic pattern, the system comprising:
 a mold, the mold comprising microfluidic channels, the microfluidic channels having an inlet and an outlet, the outlets of the channels configured to form part of the predetermined pattern, and   a support suitable to be coupled with the mold.   
     
     
         31 . The system of  claim 30 , wherein said pattern comprises substantially parallel lines forming a barcoded pattern. 
     
     
         32 - 33 . (canceled)