US2016002652A1PendingUtilityA1
Transcription factors for increasing yield
Est. expiryNov 17, 2019(expired)· nominal 20-yr term from priority
Inventors:Robert A. CreelmanLuc AdamJose Luis RiechmannJacqueline E. HeardOmaira PinedaCai-Zhong JiangOliver J. RatcliffeT. Lynne Reuber
C12N 15/8273C12N 15/8201C07K 14/415C12N 15/8261C12N 15/8282C12N 15/8293C12N 15/8251C12N 15/827C12N 15/8269Y02A40/146C12N 15/8241C12N 15/8279C12N 15/8247C12N 15/8271C12N 15/8266
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Claims
Abstract
The invention is directed to transgenic plants transformed with nucleic acids that encode a plant transcription factor that increases the transgenic plant's size and yield and/or delays flowering in the plant, and methods of using and producing the transgenic plants.
Claims
exact text as granted — not AI-modifiedWhat is claimed is:
1 . A transgenic plant having an improved trait relative to a control plant, wherein:
the transgenic plant comprises a recombinant polynucleotide that encodes a polypeptide;
wherein the polypeptide has an amino acid identity to the full length of a listed polypeptide sequence; or
the polypeptide comprises a conserved domain has an amino acid identity to a conserved domain of a listed polypeptide sequence;
and the listed polypeptide sequence is selected from the group consisting of SEQ ID NOs: 110, 112, 116, 120, 124, 128, 131, 135, 139, 143, 147, 151, 155, 159, 163, 167, 171, 175, 179, 183, 187, 191, 195, 199, 203, 207, 211, 215, 219, 223, 227, 231, 235, 239, 243, 247, 251, 255, 259, 263, 267, 271, 275, 280, 284, 288, 292, 296, 299, 303, 306, 309, 313, 317, 321, 325, 329, 333, 337, 341, 345, 349, 353, 357, 361, 365, 369, 373, 377, 381, 385, 389, 393, 397, 401, 404, 406, 409, 413, 416, 419, 422, 425, 428, 431, 435, 439, 443, 447, 451, 454, 458, 462, 465, 468, 471, 475, 478, 482, 485, 489, 493, 497, 501, 505, 509, 512, 515, 519, 522, 526, 530, 534, 538, 542, 546, 550, 553, 557, 561, 565, 568, 571, 574, 577, 581, 585, 588, 591, 594, 597, 601, 605, 609, 613, 616, 620, 624, 628, 632, 636, 640, 644, 648, 652, 656, 660, 664, 667, 671, 674, 678, 682, 686, 689, 692, 696, 700, 704, 708, 712, 715, 719, 723, 727, 731, 734, 738, 741, 745, 749, 752, 756, 760, 762, 766, 770, 774, 778, 782, 786, 789, 793, 797, 801, 805, 809, 813, 816, 819, 823, 827, 831, 835, 839, 843, 847, 851, 855, 859, 863, 867, 871, 874, 878, 882, 886, 890, 894, 898, 901, 905, 909, 913, 917, 921, 925, 929, 933, 937, 941, 945, 949, 953, 957, 960, 963, 966, 970, 973, 976, 980, 984, 988, 992, 995, 999, 1003, 1007, 1011, 1015, 1019, 1023, 1027, 1031, 1037, 1041, 1045, 1049, 1052, 1056, 1060, 1064, 1067, 1071, 1075, 1078, 1081, 1085, 1089, 1093, 1097, 1101, 1104, 1108, 1112, 1116, 1120, 1123, 1126, 1130, 1134, 1138, 1142, 1145, 1148, 1151, 1154, 1157, 1161, 1165, 1169, 1173, 1177, 1181, 1185, 1188, 1192, 1195, 1199, 1203, 1207, 1211, 1215, 1219, 1222, 1226, 1229, 1233, 1236, 1240, 1243, 1247, 1251, 1254, 1258, 1262, 1266, 1269, 1273, 1277, 1281, 1285, 1289, 1293, 1297, 1300, 1304, 1308, 1311, 1314, 1318, 1322, 1326, 1330, 1334, 1338, 1342, 1346, 1350, 1354, 1358, 1361, 1365, 1369, 1372, 1376, 1380, 1384, 1388, 1392, 1396, 1400, 1404, 1408, 1411, 1415, 1419, 1423, 1427, 1431, 1435, 1439, 1443, 1446, 1449, 1452, 1455, 1459, 1463, 1467, 1470, 1474, 1477, 1481, 1488, 1492, 1495, 1499, 1503, 1507, 1511, 1515, 1519, 1522, 1526, 1530, 1533, 1537, 1541, 1545, 1549, 1553, 1557, 1561, 1565, 1568, 1572, 1576, 1579, 1583, 1586, 1589, 1593, 1596, 1598, 1602, 1604, 1608, 1611, 1614, 1617, 1620, 1624, 1628, 1632, 1636, 1640, 1645, 1648, 1652, 1656, 1660, 1664, 1668, 1672, 1676, 1680, 1684, 1688, 1692, 1696, 1700, 1704, 1707, 1711, 1715, 1719, 1722, 1726, 1729, 1733, 1737, 1741, 1745, 1749, 1753, 1757, 1761, 1765, 1769, 1773, 1777, 1781, 1785, 1789, 1793, 1796, 1800, 1803, 1806, 1809, 1812, 1816, 1820, 1824, 1827, 1831, 1835, 1838, 1841, 1844, 1846, 1850, 1853, 1857, 1861, 1865, 1869, 1873, 1877, 1881, 1885, 1889, 1893, 1897, 1901, 1904, 1908, 1912, 1916, 1920, 1924, 1928, 1932, 1935, 1939, 1943, 1949, 1957, 1961, 1964, 1967, 1970, 1973, 1977, 1981, 1984, 1986, 1988, 1990, 1992, 1994, 1996, 1998; and 1999-2007;
wherein the amino acid identity is selected from the group consisting of at least 46%, or at least 50%, at least 53%, at least 56%, at least 61%, at least 80%, at least 85%, at least 90%, at least 91%, at least 97%, or about 100%;
wherein the altered trait is selected from the group consisting of: greater yield, larger size, larger seeds, increased root mass, reduced trichome number, increased trichome density and size, darker green color, increased rate of photosynthesis, later senescence, more tolerance to water deprivation, more tolerance to hyperosmotic stress, more drought tolerance, less sensitive to abscisic acid, more heat tolerance, more tolerance to salt, more tolerance to glucose, more tolerance to mannitol, more cold tolerance, more tolerance to nitrogen-limited conditions, early flowering, delayed flowering, more resistance to disease, more resistance to a fungal disease, more resistance to Botrytis , more resistance to Erysiphe , more resistance to Sclerotinia , increased shade tolerance, more seed protein, and more seed oil relative to the control plant that does contain the recombinant polynucleotide.
2 . The transgenic plant of claim 1 , wherein the recombinant polynucleotide comprises a constitutive, inducible, or tissue-specific promoter.
3 . The transgenic plant of claim 1 , wherein the transgenic plant is a transgenic seed comprising the conserved domain of claim 1 .
4 . The transgenic plant of claim 1 , wherein said transgenic plant is a Leguminosae, alfalfa, soybean, clover, Umbelliferae, carrot, celery, parsnip, Cruciferae, cabbage, radish, rapeseed, broccoli, Curcurbitaceae, melon, cucumber, Gramineae, wheat, corn, rice, barley, millet, Solanaceae, potato, tomato, tobacco, or pepper plant.
5 . A method for producing and identifying a transgenic plant having an altered trait relative to a control plant, the method steps comprising the steps of:
(a) introducing into a target plant a recombinant polynucleotide that encodes a polypeptide;
wherein the polypeptide has an amino acid identity to the full length of a listed polypeptide sequence; or
the polypeptide comprises a conserved domain has an amino acid identity to a conserved domain of a listed polypeptide sequence;
and the listed polypeptide sequence is selected from the group consisting of SEQ ID NOs: 110, 112, 116, 120, 124, 128, 131, 135, 139, 143, 147, 151, 155, 159, 163, 167, 171, 175, 179, 183, 187, 191,195,199,203,207,211,215,219,223,227,231,235,239,243,247,251,255,259,263,267, 271, 275, 280, 284, 288, 292, 296, 299, 303, 306, 309, 313, 317, 321, 325, 329, 333, 337, 341, 345, 349, 353, 357, 361, 365, 369, 373, 377, 381, 385, 389, 393, 397, 401, 404, 406, 409, 413, 416, 419, 422, 425, 428, 431, 435, 439, 443, 447, 451, 454, 458, 462, 465, 468, 471, 475, 478, 482, 485, 489, 493, 497, 501, 505, 509, 512, 515, 519, 522, 526, 530, 534, 538, 542, 546, 550, 553, 557, 561, 565, 568, 571, 574, 577, 581, 585, 588, 591, 594, 597, 601, 605, 609, 613, 616, 620, 624, 628, 632, 636, 640, 644, 648, 652, 656, 660, 664, 667, 671, 674, 678, 682, 686, 689, 692, 696, 700, 704, 708, 712, 715, 719, 723, 727, 731, 734, 738, 741, 745, 749, 752, 756, 760, 762, 766, 770, 774, 778, 782, 786, 789, 793, 797, 801, 805, 809, 813, 816, 819, 823, 827, 831, 835, 839, 843, 847, 851, 855, 859, 863, 867, 871, 874, 878, 882, 886, 890, 894, 898, 901, 905, 909, 913, 917, 921, 925, 929, 933, 937, 941, 945, 949, 953, 957, 960, 963, 966, 970, 973, 976, 980, 984, 988, 992, 995, 999, 1003, 1007, 1011, 1015, 1019, 1023, 1027, 1031, 1037, 1041, 1045, 1049, 1052, 1056, 1060, 1064, 1067, 1071, 1075, 1078, 1081, 1085, 1089, 1093, 1097, 1101, 1104, 1108, 1112, 1116, 1120, 1123, 1126, 1130, 1134, 1138, 1142, 1145, 1148, 1151, 1154, 1157, 1161, 1165, 1169, 1173, 1177, 1181, 1185, 1188, 1192, 1195, 1199, 1203, 1207, 1211, 1215, 1219, 1222, 1226, 1229, 1233, 1236, 1240, 1243, 1247, 1251, 1254, 1258, 1262, 1266, 1269, 1273, 1277, 1281, 1285, 1289, 1293, 1297, 1300, 1304, 1308, 1311, 1314, 1318, 1322, 1326, 1330, 1334, 1338, 1342, 1346, 1350, 1354, 1358, 1361, 1365, 1369, 1372, 1376, 1380, 1384, 1388, 1392, 1396, 1400, 1404, 1408, 1411, 1415, 1419, 1423, 1427, 1431, 1435, 1439, 1443, 1446, 1449, 1452, 1455, 1459, 1463, 1467, 1470, 1474, 1477, 1481, 1488, 1492, 1495, 1499, 1503, 1507, 1511, 1515, 1519, 1522, 1526, 1530, 1533, 1537, 1541, 1545, 1549, 1553, 1557, 1561, 1565, 1568, 1572, 1576, 1579, 1583, 1586, 1589, 1593, 1596, 1598, 1602, 1604, 1608, 1611, 1614, 1617, 1620, 1624, 1628, 1632, 1636, 1640, 1645, 1648, 1652, 1656, 1660, 1664, 1668, 1672, 1676, 1680, 1684, 1688, 1692, 1696, 1700, 1704, 1707, 1711, 1715, 1719, 1722, 1726, 1729, 1733, 1737, 1741, 1745, 1749, 1753, 1757, 1761, 1765, 1769, 1773, 1777, 1781, 1785, 1789, 1793, 1796, 1800, 1803, 1806, 1809, 1812, 1816, 1820, 1824, 1827, 1831, 1835, 1838, 1841, 1844, 1846, 1850, 1853, 1857, 1861, 1865, 1869, 1873, 1877, 1881, 1885, 1889, 1893, 1897, 1901, 1904, 1908, 1912, 1916, 1920, 1924, 1928, 1932, 1935, 1939, 1943, 1949, 1957, 1961, 1964, 1967, 1970, 1973, 1977, 1981, 1984, 1986, 1988, 1990, 1992, 1994, 1996, 1998; and 1999-2007;
wherein the amino acid identity is selected from the group consisting of at least 46%, or at least 50%, at least 53%, at least 56%, at least 61%, at least 80%, at least 85%, at least 90%, at least 91%, at least 97%, or about 100%;
wherein the altered trait is selected from the group consisting of: greater yield, larger size, larger seeds, increased root mass, reduced trichome number, increased trichome density and size, darker green color, increased rate of photosynthesis, later senescence, more tolerance to water deprivation, more tolerance to hyperosmotic stress, more drought tolerance, less sensitive to abscisic acid, more heat tolerance, more tolerance to salt, more tolerance to glucose, more tolerance to mannitol, more cold tolerance, more tolerance to nitrogen-limited conditions, early flowering, delayed flowering, more resistance to disease, more resistance to a fungal disease, more resistance to Botrytis , more resistance to Erysiphe , more resistance to Sclerotinia , increased shade tolerance, more seed protein, and more seed oil relative to the control plant; and
(b) identifying the transgenic plant having the altered trait relative to a control plant that does contain the recombinant polynucleotide.
6 . The method of claim 5 , wherein said transgenic plant is a Leguminosae, alfalfa, soybean, clover, Umbelliferae, carrot, celery, parsnip, Cruciferae, cabbage, radish, rapeseed, broccoli, Curcurbitaceae, melon, cucumber, Gramineae, wheat, corn, rice, barley, millet, Solanaceae, potato, tomato, tobacco, or pepper plant.
7 . The transgenic plant of claim 5 , wherein the recombinant polynucleotide comprises a constitutive, inducible, or tissue-specific promoter.
8 . The transgenic plant of claim 5 , wherein the transgenic plant is a transgenic seed comprising the conserved domain of claim 1 .Join the waitlist — get patent alerts
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