Medicinal composition for liver diseases

Abstract

The invention provides a composition containing extracts from salvia miltiorrhiza bunge, gynostemma pentaphyllummak, Chinese magnoliavine, cordyceps extract piece, pollen pini, and semen persicae and methods of and using the same.

1 . A composition for treating, preventing or ameliorating a liver disease, comprising
 an extract from  salvia miltiorrhiza  bunge;   an extract from  cordyceps  extract piece;   an extract from semen  persicae ; and   an extract from at least one of  gynostemma  pentaphyllummak, Chinese magnoliavine and pollen pini.   
     
     
         2 . The composition of  claim 1 , wherein the extract from  salvia miltiorrhiza  bunge has a percentage in the range between about 25 and about 40 wt %. 
     
     
         3 . The composition of  claim 1 , comprising:
 the extract from  salvia miltiorrhiza  bunge in a range between about 25 and about 40 wt %,   the extract from  gynostemma  pentaphyllummak in a range between about 20 and about 35 wt %,   the extract from Chinese magnoliavine in a range between about 5 and about 12 wt %,   the extract from  cordyceps  extract piece in a range between about 12 and about 20 wt %,   the extract from pollen pini in a range between about 5 and 12 wt %, and   the extract from semen  persicae  in a range between about 5 and about 12 wt %.   
     
     
         4 . The composition of  claim 1 , comprising:
 the extract from  salvia miltiorrhiza  bunge in about 38 wt %,   the extract from notoqinsenosides of  gynostemma  pentaphyllummak in about 25 wt %,   the extract from chinese magnoliavine in about 6 wt %,   the extract from  cordyceps  extract piece in about 19 wt %,   the extract from pollen pini in about 6 wt %, and   the extract from semen  persicae  in about 6 wt %.   
     
     
         5 . The composition of  claim 3 , wherein
 the extract from  salvia miltiorrhiza  bunge has a content of salvianolic acid 2.5-10 wt %,   the extract from fructus schisandrae  chinensis  has a content of schisandrin 0.3-2.0 wt %,   the extract from  cordyceps  extract piece has a content of adenosine 0.1 wt %-5.0 wt %, and   the extract from  gynostemma  pentaphyllummak has a content of notoqinsenosides 0.5 wt %-8.0 wt %.   
     
     
         6 . The composition of  claim 4 , wherein
 the extract from  salvia miltiorrhiza  bunge has a content of salvianolic acid 2.5-10 wt %,   the extract from fructus schisandrae  chinensis  has a content of schisandrin 0.3-2.0 wt %,   the extract from  cordyceps  extract piece has a content of adenosine 0.1 wt %-5.0 wt %, and   the extract from  gynostemma  pentaphyllummak has a content of notoqinsenosides 0.5 wt %-8.0 wt %.   
     
     
         7 . A method of preparing the composition of  claim 3 , comprising:
 1) providing a dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak, which is produced by   i) providing a mixture of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak according to a selected ratio,   ii) decocting the mixture with water to generate a blend with liquid and solid parts,   iii) allowing the liquid part to settle so as to form an upper lucid,   iv) collecting the upper lucid, and then concentrating the upper lucid so as to form a wet paste,   v) adding a volume of alcohol to the wet paste after cooling to form an alcohol solution so that the total content of alcohol is about 70 wt % of the solution,   vi) allowing the alcohol solution to settle,   vii) making filtration of the alcohol solution,   viii) removing the alcohol to form the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak;   2) providing a dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , which is produced by   i) adding an amount of alcohol to a mixture of the fermentation powder of mycelium and fructus schisandrae  chinensis,      ii) heating the alcohol to reflux for a period of time to generate an alcohol extract of the fermentation powder of mycelium and fructus schisandrae  chinensis,      iii) allowing the alcohol extract to cool to ambient temperature,   iv) collecting the alcohol extract of the fermentation powder of mycelium and fructus schisandrae  chinensis,      v) repeating the acts of i)-iv),   vi) combining the alcohol extract generated in acts of i)-iv),   vii) filtrating the alcohol extract, and   viii) removing the alcohol to generate the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis;      3) providing a dry paste comprising the extract of pollen pini, which is produced by   i) extracting an amount of pollen pini with alcohol with warming to form an alcohol extract of pollen pini,   ii) repeating the extracting of i),   iii) combining the alcohol extract, and   iv) removing the alcohol from the alcohol extract to form a dry paste comprising the extract of pollen pini;   4) mixing the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllummak, the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , and the dry paste comprising the extract of pollen pini; and   5) grounding the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllummak, the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , and the dry paste comprising the extract of pollen pini to form a grounded mixture of the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllumma, the extracts of mycelium and fructus schisandrae  chinensis , and the extract of pollen pini.   
     
     
         8 . The method of  claim 7 , further comprising:
 6) adding starch to grounded mixture.   
     
     
         9 . A method of preparing the composition of  claim 4 , comprising:
 1) providing a dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak, which is produced by   i) providing a mixture of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak according to a selected ratio,   ii) decocting the mixture with water to generate a blend with liquid and solid parts,   iii) allowing the liquid part to settle so as to form an upper lucid,   iv) collecting the upper lucid, and then concentrating the upper lucid so as to form a wet paste,   v) adding a volume of alcohol to the wet paste after cooling to form an alcohol solution so that the total content of alcohol is about 70 wt % of the solution,   vi) allowing the alcohol solution to settle,   vii) making filtration of the alcohol solution,   viii) removing the alcohol to form the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae , and  gynostemma  pentaphyllummak;   2) providing a dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , which is produced by   i) adding an amount of alcohol to a mixture of the fermentation powder of mycelium and fructus schisandrae  chinensis,      ii) heating the alcohol to reflux for a period of time to generate an alcohol extract of the fermentation powder of mycelium and fructus schisandrae  chinensis,      iii) allowing the alcohol extract to cool to ambient temperature,   iv) collecting the alcohol extract of the fermentation powder of mycelium and fructus schisandrae  chinensis,      v) repeating the acts of i)-iv),   vi) combining the alcohol extract generated in acts of i)-iv),   vii) filtrating the alcohol extract, and   viii) removing the alcohol to generate the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis;      3) providing a dry paste comprising the extract of pollen pini, which is produced by   i) extracting an amount of pollen pini with alcohol with warming to form an alcohol extract of pollen pini,   ii) repeating the extracting of i),   iii) combining the alcohol extract, and   iv) removing the alcohol from the alcohol extract to form a dry paste comprising the extract of pollen pini;   4) mixing the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllummak, the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , and the dry paste comprising the extract of pollen pini; and   5) grounding the dry paste comprising the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllummak, the dry paste comprising the extracts of mycelium and fructus schisandrae  chinensis , and the dry paste comprising the extract of pollen pini to form a grounded mixture of the extracts of  salvia miltiorrhiza  bunge, semen  persicae, gynostemma  pentaphyllumma, the extracts of mycelium and fructus schisandrae  chinensis , and the extract of pollen pini.   
     
     
         10 . The method of  claim 9 , further comprising:
 6) adding starch to grounded mixture.   
     
     
         11 . The composition of  claim 3  in a formulation selected from tablets, capsules, granules, dispersible tablets, sustained-release tablets, dropping pills or oral solutions. 
     
     
         12 . The composition of  claim 4  in a formulation selected from tablets, capsules, granules, dispersible tablets, sustained-release tablets, dropping pills or oral solutions. 
     
     
         13 . The composition of  claim 3 , wherein the liver disease is chronic hepatitis or hepatic fibrosis. 
     
     
         14 . The composition of  claim 4 , wherein the liver disease is chronic hepatitis or hepatic fibrosis. 
     
     
         15 . A method of treating, preventing, or ameliorating a liver disease, comprising administering to a human being the composition of  claim 3 . 
     
     
         16 . A method of treating, preventing, or ameliorating a liver disease, comprising administering to a human being the composition of  claim 3 . 
     
     
         17 . The method of  claim 15 , wherein the liver disease is chronic hepatitis or hepatic fibrosis. 
     
     
         18 . The method of  claim 16 , wherein the liver disease is chronic hepatitis or hepatic fibrosis. 
     
     
         19 . The method of  claim 17 , wherein the chronic hepatitis is hepatitis B or hepatitis C. 
     
     
         20 . The method of  claim 18 , wherein herein the chronic hepatitis is hepatitis B or hepatitis C.