Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same

Abstract

The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 - 48 . (canceled) 
     
     
         49 . A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having endoglucanase activity has at least 94% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         50 . (canceled) 
     
     
         51 . A process for degrading a cellulosic material, said process comprising:
 (i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity; and   (ii) recovering the degraded cellulosic material;   wherein the polypeptide having endoglucanase activity has at least 94% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4.   
     
     
         52 . A process for producing a fermentation product, said process comprising:
 (a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoglucanase activity;   (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and   (c) recovering the fermentation product from the fermentation;   wherein the polypeptide having endoqlucanase activity has at least 94% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4.   
     
     
         53 . (canceled) 
     
     
         54 . A nucleic acid construct comprising a polynucleotide encoding a polypeptide comprising a catalytic domain, wherein the polynucleotide is operably linked to one or more heteroloqous control sequences that direct the production of the polypeptide in an expression host, wherein the catalytic domain has at least 94% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4 or comprises, a fragment of the sequence of amino acids 73 to 386 of SEQ ID NO: 4, and wherein the catalytic domain has endoglucanase activity. 
     
     
         55 . The nucleic acid construct of  claim 54 , wherein the polypeptide further comprising a cellulose binding domain. 
     
     
         56 . (canceled) 
     
     
         57 . A process for degrading a cellulosic material, said process comprising:
 (i) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoqlucanase activity; and   (ii) recovering the degraded cellulosic material;   wherein the polypeptide having endoqlucanase activity comprises a catalytic domain having at least 94% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4, or the polypeptide having endoqlucanase activity comprises a catalytic domain having a fragment of the sequence of amino acids 73 to 386 of SEQ ID NO: 4.   
     
     
         58 . A process for producing a fermentation product,
 said process comprising:   (a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having endoqlucanase activity;   (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and   (c) recovering the fermentation product from the fermentation;   wherein the polypeptide having endoqlucanase activity comprises a catalytic domain having at least 94% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4, or the polypeptide having endoqlucanase activity comprises a catalytic domain having a fragment of the sequence of amino acids 73 to 386 of SEQ ID NO: 4.   
     
     
         59 . (canceled) 
     
     
         60 . The nucleic acid construct of  claim 49 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         61 . The nucleic acid construct of  claim 49 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         62 . The nucleic acid construct of  claim 49 , wherein the polypeptide having endoglucanase activity is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         63 . The nucleic acid construct of  claim 49 , wherein the polypeptide having endoglucanase activity is a variant of the sequence of amino acids 21 to 386 of SEQ ID NO: 4, comprising a substitution, deletion and/or insertion of one or more amino acids. 
     
     
         64 . A recombinant host cell comprising the nucleic acid construct of  claim 49 . 
     
     
         65 . A method of producing a polypeptide having endoglucanase activity, said method comprising:
 (a) cultivating the host cell of  claim 64  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         66 . A recombinant host cell transformed with a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity has at least 94% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         67 . The recombinant host cell of  claim 66 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         68 . The recombinant host cell of  claim 66 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         69 . A method of producing a polypeptide having endoglucanase activity, said method comprising:
 (a) cultivating a host cell comprising a polynucleotide encoding a polypeptide having endoglucanase activity under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide;   wherein the polypeptide having endoglucanase activity has at least 94% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4, or is a fragment of the sequence of amino acids 21 to 386 of SEQ ID NO: 4.   
     
     
         70 . The process of  claim 51 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         71 . The process of  claim 51 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         72 . The process of  claim 52 , wherein the polypeptide having endoglucanase activity has at least 97% sequence identity to the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         73 . The process of  claim 52 , wherein the polypeptide having endoglucanase activity comprises the sequence of amino acids 21 to 386 of SEQ ID NO: 4. 
     
     
         74 . The nucleic acid construct of  claim 54 , wherein the catalytic domain has at least 97% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         75 . The nucleic acid construct of  claim 54 , wherein the catalytic domain comprises the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         76 . The process of  claim 57 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 97% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         77 . The process of  claim 57 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         78 . The process of  claim 58 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 97% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         79 . The process of  claim 58 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         80 . A recombinant host cell transformed with a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 94% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4, or the polypeptide having endoglucanase activity comprises a catalytic domain having a fragment of the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         81 . The recombinant host cell of  claim 80 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having at least 97% sequence identity to the sequence of amino acids 73 to 386 of SEQ ID NO: 4. 
     
     
         82 . The recombinant host cell of  claim 81 , wherein the polypeptide having endoglucanase activity comprises a catalytic domain having the sequence of amino acids 73 to 386 of SEQ ID NO: 4.