Polypeptides Having Phospholipase C Activity and Polynucleotides Encoding Same

Abstract

Provided are isolated polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . An isolated polypeptide having phospholipase C activity, selected from the group consisting of:
 (a) a polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2;   (b) a polypeptide encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, or (ii) the full-length complement of (i);   (c) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1;   (d) a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions; and   (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has phospholipase C activity.   
     
     
         2 . The polypeptide of  claim 1 , comprising or consisting of SEQ ID NO: 2 or the mature polypeptide of SEQ ID NO: 2. 
     
     
         3 . The polypeptide of  claim 1 , wherein the mature polypeptide is amino acids 1 to 594 of SEQ ID NO: 2. 
     
     
         4 . The polypeptide of  claim 1 , which is a variant of the mature polypeptide of SEQ ID NO: 2 comprising a substitution, deletion, and/or insertion at one or more positions. 
     
     
         5 . The polypeptide of  claim 1 , which is a fragment of SEQ ID NO: 2, wherein the fragment has phospholipase C activity. 
     
     
         6 . A composition comprising the polypeptide of  claim 1 . 
     
     
         7 . (canceled) 
     
     
         8 . (canceled) 
     
     
         9 . A method for reducing the content of phosphorus containing components in an edible oil, comprising contacting said oil with an aqueous solution of the polypeptide of  claim 1 , which aqueous solution is emulsified in the oil until the phosphorus content of the oil is reduced, and then separating the aqueous phase from the treated oil. 
     
     
         10 . An isolated polynucleotide encoding the polypeptide of  claim 1 . 
     
     
         11 . A nucleic acid construct or expression vector comprising the polynucleotide of  claim 10  operably linked to one or more control sequences that direct the production of the polypeptide in an expression host. 
     
     
         12 . A recombinant host cell comprising the polynucleotide of  claim 10  operably linked to one or more control sequences that direct the production of the polypeptide. 
     
     
         13 . A method of producing the polypeptide of  claim 1 , comprising:
 (a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         14 . A method of producing a polypeptide having phospholipase C activity, comprising:
 (a) cultivating the host cell of  claim 12  under conditions conducive for production of the polypeptide; and   (b) recovering the polypeptide.   
     
     
         15 . A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding the polypeptide of  claim 1 .