Methods of purifying steviol glycosides and uses of the same

Abstract

A method for purifying steviol glycosides by utilizing a plurality of columns is described.

1 - 61 . (canceled) 
     
     
         62 . A method for purifying steviol glycosides, comprising the steps of:
 a. providing a solution comprising steviol glycosides;   b. providing a plurality of consecutively connected columns packed with an adsorbent resin capable of adsorbing steviol glycosides to create a multi-column system;   c. passing the solution of steviol glycosides through the multi-column system to obtain at least two columns, each column having a different ratio of adsorbed steviol glycosides; and   d. eluting the adsorbed steviol glycosides from at least one column in the multi-column system, to obtain an eluted solution comprising steviol glycosides including Rebaudioside X.   
     
     
         63 . The method of  claim 62 , further comprising eluting high Rebaudioside X fractions from the multi-column system to obtain an eluted Rebaudioside X solution. 
     
     
         64 . The method of  claim 62 , further comprising the step of removing impurities by washing the multi-column system with a washing solution prior to eluting the adsorbed steviol glycosides. 
     
     
         65 . The method of  claim 64 , wherein the washing solution comprises an aqueous alcohol solution having a water to alcohol ratio ranging from about 99.9:0.1 to about 60:40. 
     
     
         66 . The method of  claim 62 , wherein the adsorbed steviol glycosides are eluted with an aqueous alcohol solution having a water to alcohol ratio ranging from about 60:40 to about 0.1:99.9. 
     
     
         67 . The method of  claim 62 , wherein 3 to 15 columns are consecutively connected in series. 
     
     
         68 . The method of  claim 62 , wherein a ratio of a volume of a first column of the plurality of columns to a volume of a second column of the plurality of columns is in the range of about 1:1 to about 1:10. 
     
     
         69 . The method of  claim 62 , wherein a ratio of a volume of a last column of the plurality of columns to a volume of a penultimate column of the plurality of columns is in the range of about 3:1 to about 1:10. 
     
     
         70 . The method of  claim 62 , wherein the solution of steviol glycosides is obtained by dissolving a composition comprising a steviol glycoside mixture in a solvent. 
     
     
         71 . The method of  claim 62 , further comprising the step of drying the eluted solution comprising steviol glycosides to obtain a purified steviol glycoside mixture. 
     
     
         72 . The method of  claim 62 , wherein the solution of steviol glycosides is prepared by
 a. providing a biomass of a  Stevia rebaudiana  plant;   b. producing a crude extract by contacting the biomass with water;   c. separating insoluble material from the crude extract to obtain a filtrate containing steviol glycosides; and   d. treating the filtrate to remove high molecular weight compounds and insoluble particles to obtain the solution of steviol glycosides.   
     
     
         73 . The method of  claim 62 , further comprising the steps of:
 a. decolorizing and deionizing the eluted solution comprising steviol glycosides to obtain a decolorized, deionized steviol glycoside solution; and   b. drying the decolorized, deionized steviol glycoside solution to obtain a purified steviol glycoside mixture.   
     
     
         74 . The method of  claim 62 , wherein steviol glycosides are selected from the group consisting of Rebaudioside A, Rebaudioside B, Rebaudioside C, Rebaudioside D, Rebaudioside E, Rebaudioside F, Rebaudioside X, Stevioside, Steviolbioside, Dulcoside A, Rubusoside, or other glycoside of steviol and combinations thereof. 
     
     
         75 . The method of  claim 64 , wherein the washing solution comprises at least one alkali. 
     
     
         76 . The method of  claim 64 , wherein the washing solution comprises at least one acid. 
     
     
         77 . The method of  claim 64 , wherein the washing solution comprises water.