Biologically formed nanoscale devices

Abstract

The invention in suitable embodiments is directed to biologically formed nanoscale devices and components employing in whole or in part isolated bio-nanoparticle elements. In one aspect, one or more device, formed in whole or in part from isolated, synthetic and or recombinant amino acid residues comprising in whole or in part one or more types of isolated clathrin protein and/or non-clathrin coatomer proteins of one or more isoforms, form one or one or more types and forms of nanoscale devices and/or components thereof for use in vivo or in vitro.

In view of the foregoing, what is claimed is: 
     
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         38 . An isolated protein platform up to 100 nanometers in diameter and self-assembled from one or more isolated SEQ ID NO: 1 for use as a scalable technique to assemble in whole and/or in part a nanoscale device, comprising: a selected nanoscale device configuration; one or more additional techniques for optional use in assembling the selected configuration; one or more elements for use in functionalizing the selected configuration; an energy resource capable of generating at least one form of energy and usable by the selected configuration, wherein the assembled nanoscale device is capable of utilizing at least some of the generated energy to provide one or more function in vivo or in vitro. 
     
     
         39 . The isolated protein platform according to  claim 38 , wherein the isolated SEQ ID NO: 1 is also configured with one or more isolated sequence selected from an isolated SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, and combinations thereof. 
     
     
         40 . The isolated protein platform according to  claim 38 , wherein the isolated SEQ ID NO: 1 is substituted in whole or in part with one more isolated sequence selected from an isolated SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and combinations thereof. 
     
     
         41 . The isolated protein platform according to  claim 38 , wherein the isolated SEQ ID NO: 1 is substituted in whole or in part with one more isolated sequence selected from an isolated SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, and combinations thereof. 
     
     
         42 . The isolated protein platform of any of  claims 38 - 40 , wherein said isolated sequence comprises in whole or in part a plurality of clathrin coatomer protein molecules selected from purified, isolated, synthetic, recombinant, biologically modified, chemically modified, genetically modified, hybridized, chiral clathrin coatomer protein molecules, and combinations thereof. 
     
     
         43 . The isolated protein platform according to  claim 41 , wherein said isolated sequence comprises in whole or in part a plurality of non-clathrin-coat protein molecules selected from purified, isolated, synthetic, recombinant, biologically modified, chemically modified, genetically modified, hybridized, chiral non-clathrin-coat protein molecules, and combinations thereof. 
     
     
         44 . The isolated protein platform according to  claim 38 , wherein the isolated SEQ ID NO: 1 comprises in whole or in part a purified clathrin heavy chain element. 
     
     
         45 . The isolated protein platform according to  claim 44 , wherein the purified clathrin heavy chain element comprises in whole or in part one or more interaction site elements, distinctive domain elements, heavy chain subunit elements, light chain subunit elements, and combinations thereof. 
     
     
         46 . The isolated protein platform according to  claim 38 , wherein a configuration of one or more of the isolated SEQ ID NO: 1 forms in whole or in part one or more legs of an isolated clathrin triskelion element. 
     
     
         47 . The isolated protein platform according to  claim 38 , wherein the isolated protein platform comprises one or more preparations of isolated heterotetrameric adaptor protein elements selected from an isolated AP-1, AP-2, AP-3, AP-4, AP-5, AP-6, AP-1A, AP-1B, AP-3B, b-arrestin, tubulin protein, dynamin protein, epsin protein, endophilin protein, synaptotagmin, Golgi-localizing, Gamma-adaptin ear domain homology, ARF-binding element, and combinations thereof. 
     
     
         48 . The isolated protein platform according to  claim 38 , wherein the isolated protein platform is greater than one nanometer in diameter. 
     
     
         49 . The isolated protein platform according to  claim 38 , wherein the isolated protein platform is at least about 50 nanometers in diameter. 
     
     
         50 . The isolated protein platform according to  claim 38 , wherein the isolated protein platform is at least about 100 nanometers in diameter. 
     
     
         51 . The isolated protein platform according to  claim 38 , wherein the step of assembling comprises in whole or in part in vivo or in vitro self-assembly, disassembly, and/or a response of the isolated protein platform. 
     
     
         52 . The isolated protein platform according to  claim 51 , wherein the step of assembling includes use of one or more pH factor and/or a heat shock cognate protein. 
     
     
         53 . The isolated protein platform according to  claim 38 , wherein the additional technique is selected from the group consisting of an adduct, bio-molecular, biochemical, biodegradable, bioengineered, chemical, chromatographic, enzyme, externally directed, glass surface, imprint, imprinted membrane, imprinted polymer, lithographic, mechanically assembled, mechanistically directed, mimic, molded, molecular imprint, monolith, nanofabrication, print, polymer, protein-based, scalable, self-assembling, shape imprinted, shape programmable, shaped scaffolding, silica wafer, site-specific, site directed mutagenesis, structured, surface imprint, template, thin film technique, and combinations thereof. 
     
     
         54 . The isolated protein platform according to  claim 38 , wherein the nanoscale device configuration is selected from the group consisting of an acoustic, algorithmic, analog, assay, agriculture, autonomous, battery, biochemical, biochip, biological, biomedical, biomolecular, Casimir, cell control, cell communication, cell enhancing, cell regenerating, cell regulation, cell repair, cell replacement, cell supplement, chemical, communication, control, Coulomb blockade, cybernetic, cytometry, digital, delivery, diagnostic, dosing, drug, electrical, electronic component, electrical current, electromagnetic, enhancement, energy conduction, energy conversion, energy producing, energy storage, environmental, extracellular, enzyme, fabrication, genetic, goal directed, imaging, immunoassay, individualized, information processing, intelligent, intracellular, in situ, in vivo, in vitro, laser, medical, memory, metabolic, microplate analysis, microscopy, monitoring, nucleic acid-based diagnostic, nutritional, optical, phenotype, photoelectric, photonic, prosthetic, quantum mechanical, radiation, remediation, repair, replacement, scalable, self-adapting, self-directed, self-regenerating, self-regulating, self-repairing, self-replicating, solvent, recombinant, sensor, sequestration, signaling, structured, supplement, supply, synthetic, therapeutic, thermal, transduction, transmission, transport, tunable configuration, and combinations thereof. 
     
     
         55 . The isolated protein platform according to  claim 38 , wherein the energy resource is selected from the group consisting of an acoustical, biochemical, bioluminescent, biological, chemical, laser, electrical, electrical field, electromagnetic, enzyme, light emitting diode, luminescent, magnetic field, mechanical, metabolic, pH, ordinary light, photoisomerisable species, optoelectronic, photodetector, photoelectric, photonic, photosensitive, photovoltaic, quantum dot, quantum mechanical, thermal energy resource, and combinations thereof. 
     
     
         56 . The isolated protein platform according to  claim 38 , wherein the functionalization element is selected from the group consisting of an active, amino acid, biological, capsid, carbon, chemical, circuit, conductor, control, crystal, diagnostic, dendrimer, DNA, electrical, electromagnetic, endohedral, ferroelectric, ferromagnetic, fluorescent, fluid, Fullerene, gas, geometrically derived, hydrophilic, hydrophobic, imaging, in situ, in vitro, in vivo, insulator, label, large molecule, linear, liposome, liquid, macromolecule, magnetic, magnetic domain, magnetic ring, media, medicament, metal, metalloprotein, micelle, micro-electromechanical, nanoparticle, nanotube, nonlinear, optical, opto-electronic, paramagnetic, passive, peptide, pH, photonic, polarized, polymer, protein, quantum mechanical, RNA, RNA variant, radioactive, regulating, semiconductor, sensor, small molecule, storage, switch, temperature, transistor, transition metal, vapor element, and combinations thereof. 
     
     
         57 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in an electron conduction and/or transfer system. 
     
     
         58 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in an electrically or optically induced and/or controlled color change system. 
     
     
         59 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in an electronic switching system. 
     
     
         60 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in an electrical or photonic energy storage system. 
     
     
         61 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in an optical or an electrical signal amplification system. 
     
     
         62 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for arrangement in a data processing, storage, and/or transmission system. 
     
     
         63 . The isolated protein platform according to  claim 45 , wherein the step of assembling optionally or exclusively comprises configuring one or more of the purified clathrin heavy chain elements for use as functionalization elements arrangement in an operational system. 
     
     
         64 . The isolated protein platform according to  claim 38 , wherein one or more of the functionalization elements are fused with the isolated protein platform. 
     
     
         65 . The isolated protein platform according to  claim 38 , wherein one or more of the functionalization elements are hybridized with the isolated protein platform. 
     
     
         66 . The isolated protein platform according to  claim 38 , wherein one or more of the functionalization elements are bound to the isolated protein platform by a linker. 
     
     
         67 . The isolated protein platform according to  claim 38 , wherein a masking moiety is bound to any of the components by a linker. 
     
     
         68 . The isolated protein platform according to  claim 38 , wherein a targeting moiety is bound to any of the components by a linker. 
     
     
         69 . The isolated protein platform of any of  claims 66 - 68 , wherein said linker comprises one or more linkers selected from the group consisting of amino acid, biotin-avidin coupler, chemical, covalent bond, non-covalent bond, cross-linker, cysteamine, lysine, ionic, molecular bridge, PEG, peptide, molecular bridge, molecular tether, spacer, and combinations thereof. 
     
     
         70 . The isolated nanoscale platform according to  claim 38 , wherein the components and/or the nanoscale device respond in whole or in part to an internal and/or external event. 
     
     
         71 . The isolated nanoscale platform according to  claim 70 , wherein the event comprises one or more stimulus of any type, including but not limited to acoustical, biochemical, biological, chemical, covalent, disease, disorder, electrical, electromagnetic, fluidic, genotype, ionic, magnetic, mechanical, metabolic, non-covalent, optical, pathogen, pathology, pH, phenotype, photonic, quantum mechanical, radioactive, radiological, sonic, temperature, toxin stimuli type and combinations thereof. 
     
     
         72 . The isolated nanoscale platform according to  claim 70 , wherein the response comprises at least one step selected from an activation, algorithmic, alteration, amplification, analysis, assay, autonomous, cellular process, change of color, change of state, communication, computation, conduction, control, deactivation, decoding, detection, diagnostic, electronic, encoding, execution, inhibition, input, intermixing, labeling, measuring, optical, output, photonic, processing, programmed, promoting, reading, regulation, reporting, signal generation, storing, switching, tagging, testing, therapeutic, transduction, transfer, transmitting, treating step, and combinations thereof. 
     
     
         73 . The isolated protein platform according to  claim 38 , wherein the components and/or the nanoscale device are coated or surrounded in whole or in part with a material selected from the group consisting of coated or surrounded in whole or in part with one or more types of elements selected from biological, biomaterials, capsules, chemicals, conductors, cosurfactants, crystals, inorganic, insulators, metals, non-reflective, optical, organic, polymers, reflective, semiconductors, steric stabilizers, surfactants, synthetics, and combinations or thereof. 
     
     
         74 . The isolated nanoscale platform of  claim 38 , wherein the isolated nanoscale platform performs an action comprising the nanoscale device, the preceding action selected from enabling, enhancing, functionalizing, modifying, delivering, repurposing, transporting the nanoscale device, and combinations thereof. 
     
     
         75 . The isolated protein platform according to  claim 38 , wherein the isolated protein platform comprises multiple: functionalization elements, assembly techniques, nanoscale devices, energy resources, configurations, isolated SEQ ID NO: 1. 
     
     
         76 . The isolated protein platform according to  claim 38 , wherein the nanoscale device comprises a composition suitable for in vivo or in vitro use in a human or animal. 
     
     
         77 . The isolated protein platform according to  claim 38 , wherein the nanoscale device comprises a composition suitable for commercial or industrial use. 
     
     
         78 . The isolated protein platform according to  claim 38 , wherein the nanoscale device comprises a composition suitable for research use. 
     
     
         79 . A method for forming a nanoscale device comprising: forming an isolated protein platform up to 100 nanometers in diameter and self-assembled from one or more isolated SEQ ID NO: 1 for use as a scalable technique to assemble in whole and/or in part a nanoscale device, comprising: a selected nanoscale device configuration; one or more additional techniques for optional use in assembling the selected configuration; one or more elements for use in functionalizing the selected configuration; an energy resource capable of generating at least one form of energy and usable by the selected configuration, wherein the assembled nanoscale device is capable of utilizing at least some of the generated energy to provide one or more function in vivo or in vitro.