Methods and compositions for modulation of olfml3 mediated angiogenesis

Abstract

The present invention relates to antibodies against specific domains of Olfml3 and the use of such in angiogenesis. In particular aspects, angiogenesis-related conditions, such as cancer, can be treated by the composition comprising the Olfml3 antagonists.

1 - 18 . (canceled) 
     
     
         19 . A method of producing an antibody comprising administering to a subject a peptide comprising (i) an epitope within amino acid positions 86-99 of SEQ ID NO:1 (human Olfml3 protein), or (ii) an epitope within amino acid positions 390-403 of SEQ ID NO:1 (human Olfml3 protein). 
     
     
         20 . The method of  claim 19 , further comprising obtaining a B-cell expressing said antibody. 
     
     
         21 . The method of  claim 20 , further comprising immortalizing said B-cell. 
     
     
         22 . The method of  claim 21 , further comprising culturing said immortalized B-cell to produce a monoclonal antibody. 
     
     
         23 . The method of  claim 19 , wherein the antibody recognizes amino acid positions 86-99. 
     
     
         24 . The method of  claim 19 , wherein the antibody recognizes amino acid positions 390-403. 
     
     
         25 . The method of  claim 20 , wherein said subject is a mouse. 
     
     
         26 . The method of  claim 25 , further comprising humanizing said mouse antibody. 
     
     
         27 . The method of  claim 20 , wherein said subject is a human. 
     
     
         28 . The method of  claim 19 , further comprising obtaining polyclonal sera reactive with said peptide from said subject. 
     
     
         29 . The method of  claim 28 , wherein said polyclonal sera is human polyclonal sera.