Analysis Device

Abstract

An analysis device is disclosed which includes an electron detection medium to obtain information needed for analyzing an analyte in correlation with an electron transfer level, and a reagent part which is disposed on the electron detection medium and includes an electron transporting substance to transport electrons between the analyte and the electron detection medium, the electron transporting substance including a water-soluble aromatic heterocycle compound, and being free of a metal complex. An analysis method using the analysis device is also disclosed.

1 . An analysis device comprising:
 an electron detection medium to obtain information needed for analyzing an analyte in correlation with an electron transfer level; and   a reagent part which is disposed on the electron detection medium and comprises
 an oxidoreductase that transfers an electron to or from the analyte, and 
 an electron transporting substance to transport electrons between the oxidoreductase and the electron detection medium without involving any oxidation-reduction reaction, 
   wherein the electron transporting substance is a water-soluble aromatic heterocycle compound that is free of a metal complex.   
     
     
         2 . The analysis device according to  claim 1 , wherein the electron transporting substance comprises at least one water-soluble aromatic heterocycle compound selected from the group consisting of pyridine compounds and imidazole compounds. 
     
     
         3 . The analysis device according to  claim 1 , wherein the water-soluble aromatic heterocycle compound has a molecular weight of 1,000 or less. 
     
     
         4 - 5 . (canceled) 
     
     
         6 . The analysis device according to  claim 1 , wherein a concentration of the electron transporting substance is a concentration that allows transportation of electrons between the electron detection medium and the oxidoreductase. 
     
     
         7 . The analysis device according to  claim 1 , wherein a concentration of the electron transporting substance is from 10 mass % to 60 mass % based on a total mass of the reagent part. 
     
     
         8 . The analysis device according to  claim 1 , wherein the electron transporting substance comprises at least one water-soluble aromatic heterocycle compound selected from the group consisting of pyridine and aminomethyl pyridine. 
     
     
         9 . (canceled) 
     
     
         10 . The analysis device according to  claim 1 , wherein the reagent part comprises a crosslinked substance obtained by crosslinking using at least one selected from the group consisting of glutaraldehyde, carbodiimide compounds and succinimide esters. 
     
     
         11 . The analysis device according to  claim 1 , wherein the electron detection medium is an electric conductor. 
     
     
         12 . The analysis device according to  claim 1 , wherein the analyte is a saccharide. 
     
     
         13 . An analysis method comprising obtaining information needed for analyzing an analyte based on an electron transfer level of the analyte using the analysis device according to  claim 1 . 
     
     
         14 . The analysis device according to  claim 1 , wherein the electron transporting substance comprises an electron cloud of pi-electrons perpendicular to the surfaces of the molecules of the electron transporting substance. 
     
     
         15 . The analysis device according to  claim 1 , wherein the electron transporting substance comprises 4-aminomethyl pyridine or 5-amino-4-imidazolecarboxamide. 
     
     
         16 . The analysis device according to  claim 1 , wherein the electron transporting substance has a molecular weight of 800 or less. 
     
     
         17 . The analysis device according to  claim 1 , wherein the oxidoreductase and the electrode have a gap in between, and the electron transporting substance is configured to enter into the gap in the presence of the analyte. 
     
     
         18 . The analysis device according to  claim 1 , wherein
 the electron transporting substance is configured to enter into a gap between the oxidoreductase and the electrode, and   the electron transporting substance and the oxidoreductase overlap one another to construct an electron transfer pathway with pi-electrons of the electron transporting substance in the presence of the analyte.   
     
     
         19 . The analysis device according to  claim 1 , wherein the electron transporting substance is not a polymer. 
     
     
         20 . An analysis method comprising
 transporting electrons in a reagent part of an analysis device by electron transporting substances between an analyte and an electron detection medium without involving oxidation-reduction of the electron transporting substances, wherein
 the reagent part is disposed on the electron detection medium and comprises an oxidoreductase that transfers an electron to or from the analyte, and 
 the electron transporting substance is a water-soluble aromatic heterocycle compound that is free of a metal complex; and 
   obtaining information about the analyte based on an electron transfer level of the analyte by the transporting electrons step.