High speed, high fidelity, high sensitivity nucleic acid detection

Abstract

Methods, compositions, and kits for nucleic acid detection.

1 . A kit for nucleic acid detection comprising:
 a plurality of target-specific nucleic acid probes that are each complementary to a target nucleic acid, including a first target-specific nucleic acid probe, a nanorod detection probe and a second target-specific nucleic acid probe, wherein upon hybridization to the target nucleic acid the plurality of target-specific nucleic acid probes will be directly adjacent to each other;   a molecular post bound to a solid support, where the molecular post binds to the first target-specific nucleic acid probe, the nanorod detection probe to the second target-specific nucleic acid probe, the first target-specific nucleic acid probe and the second target-specific nucleic acid probe are positioned at the 5′ and 3′ end, respectively, of the series of target specific nucleic acid probes and the plurality of target-specific nucleic acid probes ligate together to produce a ligation product, and where the molecular post is selected from the group consisting of F1-ATPases, actomyosin, ciliary axonemes, bacteria flagellar posts, kinesin/microtubules, and nucleic acid helicases; and   exonuclease or a denaturing agent or both for treating the ligation product.   
     
     
         2 - 3 . (canceled) 
     
     
         4 . The kit of  claim 1 , wherein the solid support comprises a glass coverslip. 
     
     
         5 - 9 . (canceled) 
     
     
         10 . The kit of  claim 1 , wherein the nanorod detection probe comprises a gold nanorod. 
     
     
         11 . The kit of  claim 4 , wherein the nanorod detection probe comprises a gold nanorod.