Nucleic acid amplification method

Abstract

A nucleic acid amplification method includes: forming a first liquid droplet containing a first reaction reagent for amplifying a first nucleic acid in one vessel containing a silicone oil to which a fluoro-modified silicone resin is added; forming a second liquid droplet containing a second reaction reagent for amplifying a second nucleic acid in the one vessel; and performing a nucleic acid amplification reaction in the vessel by fitting the one vessel in a nucleic acid amplification reaction apparatus while maintaining the first liquid droplet independent of the second liquid droplet and then operating the nucleic acid amplification reaction apparatus.

What is claimed is: 
     
         1 . A method of performing multiple nucleic acid amplification reactions in a single common vessel, the method comprising:
 forming a first liquid droplet containing a first reaction reagent for amplifying a first nucleic acid in one vessel containing a silicone oil to which a fluoro-modified silicone resin is added;   forming a second liquid droplet containing a second reaction reagent for amplifying a second nucleic acid in the one vessel; and   performing a nucleic acid amplification reaction in the one vessel by fitting the vessel to a nucleic acid amplification reaction apparatus while maintaining the first liquid droplet independent of the second liquid droplet and then operating the nucleic acid amplification reaction apparatus.   
     
     
         2 . The method according to  claim 1 , wherein the first liquid droplet and the second liquid droplet contain the same type of nucleic acid sample. 
     
     
         3 . The method according to  claim 1 , wherein the first liquid droplet and the second liquid droplet contain different types of nucleic acid samples. 
     
     
         4 . The method according to  claim 1 , wherein the fluoro-modified silicone resin is XS66-C1191. 
     
     
         5 . The method according to  claim 1 , wherein
 the first liquid droplet contains a first fluorescent dye, and   the second liquid droplet contains a second fluorescent dye which is different from the first fluorescent dye.   
     
     
         6 . The method according to  claim 1 , wherein the additive amount of the fluoro-modified silicone resin is 1% by mass or more and 50% by mass or less. 
     
     
         7 . A vessel configured to perform multiple nucleic acid amplification reactions, wherein the vessel contains:
 a silicone oil to which a fluoro-modified silicone resin is added;   a first liquid droplet containing a reaction reagent for amplifying a first nucleic acid; and   a second liquid droplet containing a reaction reagent for amplifying a second nucleic acid.   
     
     
         8 . The vessel according to  claim 7 , wherein the fluoro-modified silicone resin is XS66-C1191. 
     
     
         9 . The vessel according to  claim 7 , wherein
 the first liquid droplet contains a first fluorescent dye, and   the second liquid droplet contains a second fluorescent dye which is different from the first fluorescent dye.   
     
     
         10 . The vessel according to  claim 7 , wherein the additive amount of the fluoro-modified silicone resin is 1% by mass or more and 50% by mass or less. 
     
     
         11 . A nucleic acid amplification apparatus, comprising:
 a body; and   a fitting section supported by the body,   wherein the vessel according to  claim 7  is fitted to the fitting section.   
     
     
         12 . A nucleic acid amplification apparatus, comprising:
 a body; and   a fitting section supported by the body,   wherein the vessel according to  claim 8  is fitted to the fitting section.   
     
     
         13 . A nucleic acid amplification apparatus, comprising:
 a body; and   a fitting section supported by the body,   wherein the vessel according to  claim 9  is fitted to the fitting section.   
     
     
         14 . A nucleic acid amplification apparatus, comprising:
 a body; and   a fitting section supported by the body,   wherein the vessel according to  claim 10  is fitted to the fitting section.